Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Molecular Cell Biology》 2009-01
Add to Favorite Get Latest Update

ESTABLISHMENT OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION FOR DETECTION OF Vibrio alginolyticus

DING Wen Chao1 HU Jian Rao2 SHI Yu Hong1 LI Ming Yun1 CHEN Jiong1(1Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211;2College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310036)  
Vibrio alginolyticus is the main Vibrio pathogen in aquaculture in the south of China.A one step loop-mediated isothermal amplification(LAMP) assay was developed for detec-tion of Vibrio alginolyticus.A set of primers were designed from the OmpK sequence of Vibrio alginolyticus.The assay was optimised to amplify Vibrio alginolyticus DNA by incubation at 65℃ for only 1 h,and required only a simple water bath or heating block to provide a constant tem-perature of 65℃.LAMP amplification products had a ladder-like appearance when electrophoresed on an agarose gel.The detection limit of the LAMP assay was n(cell)=38/mL which was found to be higher than the commonly used PCR method.The assay was evaluated using clinical samples and the results indicated the suitability and simplicity of the test as a rapid,field diagnostic tool for Vibrio alginolyticus.
【Fund】: 长江学者和创新团队发展计划(IRT0734);; 国家科技支撑计划(2007BAD43B08);; 浙江省科技厅重点项目(2008C22049);; 宁波市科技局项目(2007C10081)~~
【CateGory Index】: S941
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 PANG Xing-hong,ZHOU Yong-can,XU Xian-dong,GUO Shao-hua,XIE Zhen-yu(College of Marine Science,Experimental Teaching Demonstration Center of Marine Biology;Hainan University;Key Laboratory of Tropic Biological Resources,Ministry of Education;Hainan Key Laboratory of Tropical Hydrobiology Technology,Haikou 570228,China);Establishment of a Method for Detection of Vibrio alginolyticus Virulent Strains Based on a New Gene[J];Fisheries Science;2011-06
2 LIU Jin-ye1,2,FENG Juan1,LIU Guang-feng1,SU You-lu1,XU Li-wen1,GUO Zhi-xun1,WANG Yun-xin3(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China; 2.College of Fisheries and Life sciences Shanghai Ocean University,Shanghai 201306,China; 3.Guangdong Aquatic Animal Disease Prevention Control Center,Guangzhou 510300,China);Rapid detection of Vibrio carchariae by loop-mediated isothermal amplification[J];Guangdong Agricultural Sciences;2012-03
3 LI Yu-feng,LI Shuai,HUANG Li-juan,ZHAO Li,HUANG Dan (School of Bioengineering,Xihua University,Chengdu 610039 China);Advance of the Research on Testing of Pathogenic Microbe in Food by LAMP Technology[J];Journal of Xihua University(Natural Science Edition);2011-01
4 ZHANG Jing1,2,SHI Hui2,XIE Jian-jun2,XU Wen-jun1,2(1.Zhejiang Ocean University,Zhoushan 316000,China;2.Marine and Fisheries Research Institute of Zhejiang Province,Zhoushan 316100,China);Rapid detection of pathogenic Vibrio harveyi by loop-mediated isothermal amplification (LAMP) method[J];Journal of Shanghai Ocean University;2010-05
5 Ying Qi1 Li Taiwu1,2 Su Xiurong1 Li Ye1 Zhou Jun1 Cui Jing1 Wang Zhonghua1 Li Songwei1 (1Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211;2Ningbo City College of Vocational Technology,Ningbo 315110);Establishment of Loop-mediated Isothermal Amplification Method for Detection of Bacillus pumilus[J];Biotechnology Bulletin;2011-02
6 XUE Li-li;PANG Huan-ying;HUANG Yu-cong;WU Zao-he;JIAN Ji-chang;ZHOU Ze-jun;Fisheries College of Guangdong Ocean University;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals;Key Laboratory of Disease Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions;Zhongkai University of Agriculture and Engineering;;Prokaryotic Expression and Purification of the Recombinant Hemin-Binding Protein(HutB) of Vibrio alginolyticus Strain HY9901[J];Journal of Guangdong Ocean University;2014-03
7 Wang Yaohuan;Wang Ruina;Zhou Qianjin;Chen Jiong;Laboratory of Biochemistry and Molecular Biology,Ningbo University;;Rapid Detection of Vibrio vulnificus by Loop-mediated Isothermal Amplification Combined with Lateral Flow Dipstick Assay[J];Biotechnology Bulletin;2014-06
【Citations】
Chinese Journal Full-text Database 5 Hits
1 YAN Qing-pi WANG Jun SU Yong-quan PI Ling-bao LIU Cheng-rong (Department,of Oceanography & Institute of Subtropical Oceanography, Xiamen University 361005) Department of Aquaculture, Jimei University, Xiamen 361021) (Senior Technical Training School of;STUDIES ON ELISA METHOD FOR DETECTING Vibrio alginolyticus, THE PATHOGENIC BACTERIA OF Pseudosciana crocea[J];Marine Sciences;2001-09
2 LIU Qi LI Hai-yan WANG Qun LIU Ping DAI Fang-yu LI Jian(Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture,YellowSea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Qingdao 266071);Identification and phylogenetic analysis of a strain of Vibrio alginolyticus,a pathogen in Portunus trituberculatus with toothpaste disease[J];Marine Fisheries Research;2007-04
3 SONG Xiao ling, HUANG Jie, SHI Cheng yin (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071,China);Production and application of monoclonal antibodies of Vibrio alginolyticus[J];Journal of Fisheries of China;2001-06
4 CHENG Tian-yin,LIU Xun,CHANG Xiao-bin(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China);Establishment and application of loop-mediated isothermal amplification for detection of Aeromonas hydrophila[J];Veterinary Science in China;2007-12
5 YANG Zhi-hui~(1,2),LI Ning-qiu~(1),BAI Jun-jie~(1),SHI Cun-bin~(1),PAN Hou-jun~(1),YE Xing~(1),LAO Hai-hua~(1),WU Shu-qin~(1)(1.Pearl River Fishery Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China;2.College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China);Sequence analysis on OmpK genes and antigenicity of pathogentic Vibrios isolated from marine fishes[J];Journal of Fishery Sciences of China;2006-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHONG He1,LIU Xing2,3,GAO Xiang-gang3,LI Yun-feng3,HE Chong-bo3(1.Liaoning Entry-exit Inspection and Quarantine Bureau,Dalian 116001,China;2.College of Life Science and Technology,Dalian Fisheries University,Dalian 116023,China;3.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Laboratory of Marine Fishery Molecular Biology,Liaoning Marine Environmental Monitoring General Station,Dalian 116023,China);16S rRNA Technique and Its Application in Aquaculture[J];Fisheries Science;2009-04
2 PAN Xiao-yi,SHEN Jin-yu,YIN Wen-lin,CHAO Zheng (Key Laboratory of Fish Health and Immunology,Chinese Academy of Fishery Science,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China);Vibriosis and their mechanisms in aquatic animals[J];Journal of Dalian Fisheries University;2006-03
3 WANG Bin,FAN Wei,LI Yan,DING Jian-feng,SUN Cen, LIU Yong-bo(Key Laboratory of Mariculture & Biotechnology,Agriculture Ministry,PRC,Dalian Fisheries Univ.,Dalian 116023,China);Tow rapid ELISA methods for detection of pathogenic bacteria of hemorrhagic septicemia in cultured turbot Scophthalmus maximus[J];Journal of Dalian Fisheries University;2008-04
4 JING Hong-li,LI Qiang,WU Qiu-xian,LI Hua(Key Laboratory of Mariculture,Agriculture Ministry,PRC,Dalian Ocean University,Dalian 116023,China);Establishment of monoclonal antibody mediated indirect ELISA for detection of Shewanella smarisflavi[J];Journal of Dalian Ocean University;2010-06
5 SHAN Xiao-feng1,GUO Wei-sheng1,CHEN Chang2,QIAN Ai-dong1 (1.College of Animal Science and Technology,Jilin Agriculturral University,Changchun,Jilin,130118,China;2.Tieling Entry-Exit Inspection and Quarantine Bureau,Tieling,Liaoning,112000,China);Progress on Detection Methods for Aeromonas hydrophila[J];Progress in Veterinary Medicine;2010-05
6 Hu Huogeng,Wang Chengzhu,Chen Changfu;Immune response of bluntnose black bream to outer membrane protein of three pathogenic bacteria[J];Feed Industry;2010-12
7 Wu Bin (The Freshwater Fisheries Research Institute of Fujian Province,Fuzhou,350002,China);Indirect Enzyme-Linked Immunosorbent Assay (ELISA) for Rapid Detection of Aeromonas caviae[J];Journal of Fujian Fisheries;2006-04
8 WANG Fan(Fujian Provincial Aquatic Technolgy Extension Station,Fuzhou 350003, China);Application of Monoclonal Antibody to Aquaculture[J];Journal of Fujian Fisheries;2007-02
9 LIN Hai-ying1,MA Zhen-ning1,ZHENG Yun-quan2,GUO Yang-hao1,2,TANG Feng-xiang2(1.Institute of Pharmaceutical Biotechnology and Bioengineering,Fuzhou University,Fuzhou,Fujian 350002,China; 2.Fujian Key Lab of Medical Instrumentation and Pharmaceutical Technology,Fuzhou,Fujian 350002,China);Screening and cross-immunogenicity of broad-spectrum outer membrane protein from Vibrio[J];Journal of Fuzhou University(Natural Science Edition);2012-04
10 LIU Jin-ye1,2,FENG Juan1,LIU Guang-feng1,SU You-lu1,XU Li-wen1,GUO Zhi-xun1,WANG Yun-xin3(1.South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China; 2.College of Fisheries and Life sciences Shanghai Ocean University,Shanghai 201306,China; 3.Guangdong Aquatic Animal Disease Prevention Control Center,Guangzhou 510300,China);Rapid detection of Vibrio carchariae by loop-mediated isothermal amplification[J];Guangdong Agricultural Sciences;2012-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 XU Hong tao 1, PIAO Chun ai 2, JIANG Zhong liang 1, WANG Wen xing 3 (1. Institute of Oceanology, Chinese Academy of Sciences, Shandong, Qingdao 266071, China; 2. TAMU, TX 77843, USA; 3. First Institute of Oceanography, National Bureau of Ocean,;Study on the Causative Agent of Lymphocystic Disease in Cultured Flounder, Paralichthys Olivaceus, in Mainland China[J];CHINESE JOURNAL OF VIROLOGY;2000-03
2 NIE Kai,WANG Da-yan,QIN Meng,GAO Rong-bao,WANG Miao,ZOU Shu-mei,HAN Feng,ZHAO Xiang,LI Xi-yan,SHU Yue-long,MA Xue-jun(State Key Laboratory for molecular Virology and Genetic Engineering,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China);Colorimetric Detection of Human Influenza A H1N1 Virus by Reverse Transcription Loop Mediated Isothermal Amplification[J];Chinese Journal of Virology;2010-02
3 HE Lin1,XU Hai-sheng1,WANG Mei-zhen2,RONG Hua-nan2(1. College of Animal Science,Zhejiang University,Hangzhou 310058,China;2. Spreading Center of Technology in Aquatic Science of Cixi city of Zhejiang Province;Cixi 315300,China);Development of Rapid Detection of Infectious Hypodermal and Hematopoietic Necrosis Virus by Loop-mediated Isothermal Amplification[J];Chinese Journal of Virology;2010-06
4 MEI Guang hai,ZHU Yong sheng,CAO Rong,TANG Zhi yong (Fisheries Technical Extension Station of Haian County,Haian 226600,China);A Preliminary Study on the Bacterial Pseudotuberculosis of Wintering Tilapia[J];Fisheries Science;2002-04
5 Yang Lei,SHU Ting-fei,WEN Yan-mao(Environmental Department of ZhongShan University, Guangzhou 510275, China);Countermeasures on Sustainable Development of Mariculture in China[J];Fisheries Science;2003-04
6 JIN Shan~1, WANG Guo-liang~1, ZHAO Qin-song~1, ZHEN Tian-lun~2, CHEN Yin-er~1 (1.Life Science and Technology Faculty, Ningbo University, Ningbo 315211, China; 2.Fisheries Technical Extension Station of Zhejiang Province, Hangzhou 310012, China);Epidemiology of Vibriosis in Large Yellow Croaker Pseudosciaena crocea (Richardson) in Marine Cage Culture[J];Fisheries Science;2005-01
7 YUAN Si-ping,WANG Guo-liang,JIN Shan(College of Life-Science and Bio-technology,Ningbo University,Ningbo 315211,China);Nocardiosis in Large Yellow Croakers,Pseudosciaena Crocea Cultivated in net cages[J];Fisheries Science;2005-09
8 PENG Kai-song1,2,SHE Rui-ping2,QI Ke-zong 1,ZU Guo-zhang 1,BAO Chuan-he 1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;2.College of Veterinary Medicine,China Agricultural University,Beijing 100094,China);Adiposis Hepatica Complicating Nocardiosis in Obscure Puffer Fugu obscurus(Abe)[J];Fisheries Science;2008-12
9 ZHENG Qiu-yue1,2,CAO Ji-juan1,WANG Qiu-yan1,XU Jun-yi1,XU Yang1,FU Jun-fan2(1.Liaoning Entry-Exit Inspection & Quarantine Bureau,Dalian 116001,China;2.Shenyang Agricultural University,Shenyang 110161,China);Detection of Vibrio alginolyticus with PCR-DHPLC in Fishery Products[J];Fisheries Science;2009-03
10 WANG Bei,WU Zao-he,JIAN Ji-chang,LU Yi-shan,TANG Ju-fen,HUANG Yu-cong(Fisheries College,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Disease Controll for Aquatic Economic Animals,Guangdong Higher Education Institutions,Zhanjiang 524025,China);Optimization of Ultrasonic-plate Method for Quantitative Detection of Bacterium Vibrio alginolyticus(HY9901) Biofilm[J];Fisheries Science;2009-07
China Proceedings of conference Full-text Database 1 Hits
1 Shi-Yi Tian Shao-Ping Deng~* (Food Sensory Science Laboratory of zhejiang gongshang university,Food Safety Key Lab of Zhejiang Province,Zhejiang Gongshang University,Hangzhou 310035,E R.China);Multi-frequency Amplitude Pulse Electronic Tongue Technique and its Application[A];[C];2006
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 LU Yan-xia1,MENG Zhao-xiang2,MA Xiao-yan1,WANG Yu1,ZHANG Xian-zhou1,ZHANG Wei1(1.College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China)(2.Institute of Food Risk Management and Application,Chinese Academy of Inspection and Quarantine,Beijing 100123,China);Rapid Detection of Listeria monocytogenes in Milk Powder by Loop-mediated Isothermal Amplification[J];Modern Food Science and Technology;2012-06
2 XUAN Xiong-zhi XIE Zhen-yu ZHOU Yong-can WANG Shi-feng WU Xue-gui(Key Laboratory of Tropical Aquatic Biotechnology of Hainan Province,College of Marine Science,Hainan University,Haikou 570228);The polymorphism of lipopolysaccharides isolated from Vibrio alginolyticus strains with different virulence[J];Progress in Fishery Sciences;2011-01
3 MA Xiao-yan 1,ZHANG Yun-zhe 2,WANG Yu 1,LIU Zhe 3,WANG Xiao-li 4,ZHANG Wei 1,( 1.College of Food Science & Technology,Hebei Agriculture University,Baoding 071001,China; 2.Affiliated Hospital of Hebei University,Baoding 071000,China; 3.Science and Technology and Information College of Shijiazhuang,Shijiazhuang 050081,China; 4.Baoding Science and Technology College,Baoding 071000,China);Rapid detection of Pseudomonas cocovenenans by loop-mediated isothermal amplification[J];Science and Technology of Food Industry;2013-03
4 SUO Na,LIU Jia,LIU Mei,WANG Xue-qiang,WANG Yu-jian,XIAO Hong-mei*,ZHOU Guang-hong(National Meat Quality and Safety Control Engineering Technology Research Center,College of Food Science and Techndogy,Nanjing Agricultural University,Nanjing 210095,China);Rapid Detection of Bt Gene in Cooked Rice and Rice Wine[J];Food Science;2013-12
5 FENG Ke;HU Wen-zhong;JIANG Ai-li;Xu Yong-ping;SA-ren-gao-wa;College of Life Science and Biotechnology,Dalian University of Technology;College of Life Science,Dalian Nationalities University;;Research progress in molecular biology detection methods of Listeria monocytogenes[J];Science and Technology of Food Industry;2014-04
6 PAND Huan-ying;CHEN Li-ming;CAI Jia;TANG Ju-fen;WANG Bei;WU Zao-he;JIAN Ji-chang;Fisheries College of Guangdong Ocean University;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals;Key Laboratory of Diseases Controlling for Aquatic Economic Animals of Guangdong Higher Education Institutions;Zhongkai University of Agriculture and Engineering;;Molecular Cloning and Bioinformatics Analysis of Dihydrolipoamide Dehydrogenase Gene from Vibrio alginolyticus[J];Journal of Guangdong Ocean University;2014-01
7 HE Ping-ping1,ZHAO Yong-zhen1,CHEN Xiu-li1,ZHANG Bin1,HUANG Ting1, PENG Min1,YANG Chun-ling1,YANG Yan-hao1,LI Yong-mei1,SHAO Peng-wei1,2,CHEN Xiao-han1*(1.Guangxi Fisheries Research Institute,Guangxi Nanning 530021,China;2.College of Animal Science and Technology,Guangxi University,Guangxi Nanning 530005,China);Development of Diagnostic PCR Assay for HSP60of Vibrio harveyi[J];Southwest China Journal of Agricultural Sciences;2013-02
8 LI Jie;LU Xin;XU Jia-liang;KAN Biao;Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention;;Fast detection of Vibrio alginolyticus using SYBR green Ⅰ-based real-time PCR assay[J];Chinese Preventive Medicine;2014-03
9 LIAO Meijie;ZHANG Zheng;RONG Xiaojun;WANG Yingeng;LIU Zhichao;LUAN Jing;LI Bin;WANG Lan;CHEN Guiping;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Laboratory of Susainable Development of Marine Fisheries, Ministry of Agriculture;Shandong Entry-Exit Inspection and Quarantine Bureau of the People’s Republic of China;;Development of a SYBR Green I real-time PCR for detection of Vibrio harveyi based on the vhhP2 gene[J];Journal of Fishery Sciences of China;2014-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MAO Zhi juan,ZHUO Hua long (Ningbo Fishery Research Institute,Ningbo 315010 China);Studies on the Pathogen of the Bacteria Epidemic against Mud Crab Scylla serrata[J];Fisheries Science;2001-01
2 Cheng Tianyin etal;Study on White Point Diseuse Pathogen in Larva Turtle[J];FISHERIES SCIENCE;1995-04
3 Wu Youlu (Second Institute of Oceanography, SOA, Hangzhou, 310012)Liu Shizhong (Marine Fisheries Research Institute of Zhejiang, Zhoushan, 316000);Studies on the causes of death of Portunus trituberculatus (Miers)[J];DONGAHI MARINE SCIENCE;1995-02
4 LI Lianrui~(1,2),LU Qiang~1,HAN Wenyu~1,LIU Mingyuan~1,FU Baoquan~1(1 College of Animal Science and Veterinary Medicine,Jilin Univerinary,Changchun 130062,China;2 College of Animal Science,Tarim University,Alar 843300,China);Cloning and sequence analysis of an aer toxin gene of Aeromonas hydrophila[J];Journal of Guangxi Agricultural and Biological Science;2005-04
5 Xiao Keyu Wang Xiaoqing Zi Daorong Liu Shuqiu Jiang Wucheng(Department of Animal Sciences and Fishery, HNAC, Changsha);ISOLATION AND IDENTIFICATION OF SOFT-SHELLED TURTLE FURUNCULOSIS PATHOGEN[J];;1991-03
6 Wang Tiehui; Li Jun; Yi Yonglan; Zhou Liran ; Liu Hanqin; Lu Renhou; Chen Hongxi(Institute of Hydrobiology, Chinese Academy of Sciences,Wuhan 430072)(School of Life Sciences.Wuhan University,Wuhan 430072);DETECTION OF HEMORRHAGIC VIRUS OF GRASS CARP BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1997-01
7 Zhang Xiaohua; Xu Huaishu; Xu Bing; Ji Weishang; Han Yin;Yang Xuesong; Ma Jiankang(College of Marine ha Sciences. Ocean University of Qingdao, Qingdao 266003)(Ningbo Fisheries Research Institute, Zhejiang 315010)(Dahua Fishery Production Group Company of;STUDY ON DIAGNOSIS OF VIBROSIS IN PENAEUS CHINENSIS BY INDIRECT FLUORESCENT ANTIBODY STAINING PROCEDURE[J];OCEANOLOGIA ET LIMNOLOGIA SINICA;1997-06
8 LIN Ke-bing ; ZHOU Chen ; LIU Jia-fu; ZHOU Sheng-li; ZENG Zhi-nan; CHEN Mu (Fujian Fisheries Research Institute, Xiamen 361012 ) (Ningde Regain Frsheries Extension Station of Fujian Province, Ningde 352100);STUDIES ON THE PATHOGENIC BACTERIA OF Pseudosciaena crocea IN MARINE CAGE CULTURE[J];MARINE SCIENCES;1999-04
9 XU Li-wen LIU Guang-feng WANG Jiang-yong WANG Rui-xuan CHEN Bi-sheng* (South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300);Study on medicines control to mass mortality of larvae of Haliotis diversicolor Reeve[J];Marine Fisheries Research;2004-04
10 SHI Hui XU Wen-jun XU Han-xiang (Marine and Fishery Research Institute of Zhejiang Ocean University, Marine and Fisheries Research Institute of Zhejiang Province, Zhoushan 316100 );Studies on pathological lesion of Portunus trituberculatus artificially infected by candida oleophila[J];Marine Fisheries Research;2005-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved