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《Joural of Biology》 2001-04
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Fermentation study on the engineering bacterial of recombinant human thrombopoietin fusion protein

WEI Han-dong,XING Gui-chun,ZHANG Jin-hui, ZHANG Jun-guan,CHEN Hui-peng,HE Fu-chu (Beijing Institute of Radiation Medbicine,Beijing 100850,China)  
In this paper,fermentation condition of the engineering bacterial E.coli JM109 which expressed recombinant human thrombopoietin/glutathione (rhTPO/GST) fusion protein was investigated.Conditions that affect fermentation of the bacterial were tested and optimized.How the fermentation condition affect the quantity of whole exogenous protein the engineering bacterial expressed was also discussed.Result showed that using complex culture medium,adding organic materials and inducing with isopropyl.
【CateGory Index】: Q81
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Chinese Journal Full-text Database 3 Hits
1 Xing Guichun;Hu Zhiyuan;Yang Xiaoming;Xie Ling;He Fuchu;Wu Chutse(Beijing Institute of Radiation Medicine,Beijing 100850);cDNA Cloning and Sequencing of Human Thrombopoietin[J];HIGH TECHNOLOGY LETTERS;1996-03
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【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Fang Hongqing,Feng Erling,Shi CHuanying,Zhao Siqing,Yu Gongyi,Ma Qingjun Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071;Effect of media and dissolved oxygen on the production of subunit B of cholera toxin by recombinant Escherichia coli MM2[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1996-01
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