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《Journal of Biology》 2005-01
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Study of RAPD reaction conditions on Eucalyptus wetarensis

PAN Tian_ling~1,LI Kun_ping~2,LIU You_quan~1(1.Central South Forestry University,Zhuzhou,412006,Hunan,China;2.Guangdong College of Pharmacy,Guangzhou,510240,China)  
The RAPD reaction conditions on Eucalyptus wetarensis,such as PCR reaction mixture,PCR amplified program,and so on,were studied. The optimal reaction mixture(20μL total volume)contained 10μL genomic DNA,4.0μL ddH_2O,2.0μL 10×butter,15μL MgCl_2(25mM),035μL dNTPs(10mM),20μL primer and 015μL Taq DNA polymerase.Amplified program was as follows:preliminary denaturation(2min,92℃),followed by 35 cycles consisting of denaturation(1min,92℃),annealing(1min,36℃)and extension(2min,72℃),and a final extension(5min,72℃).
【Fund】: 湖南省自然科学基金项目(00JJY2023)
【CateGory Index】: S792
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 ShiJisen (NanjingForestryUniversity Nanjing 2 10 0 37);TheChallengestoForestryBiotechnologyandTreeBreedingin the 2 1stCentury[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-01
2 Gan Siming Shi Jisen (Laboratory of Forest Genetics and Gene Engineering Nanjing Forestry University Nanjing 210037)Bai Jiayu Xu Jianmin (Research Institute of Tropical Forestry CAF);RAPD Fingerprints of Clones of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiu-hua,WANG Wen-feng(Department of Life Science and Technology,Xinxiang Medical University,Xinxiang 453003,China);Study on Polyacrylamide Gel Electrophoresis and Silver Staining Methods for Microsatellite DNA[J];Shandong Agricultural Sciences;2009-06
2 LUO Yue-ji et al(Institute of Life science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082);Establishment and Optimization of ISSR-PCR Reaction System and Genome DNA of Discolor cinquefoil Herb[J];Journal of Anhui Agricultural Sciences;2008-03
3 ZHENG Yang-xia et al (College of Forestry and Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014);RACE Technique and Its Application in the Research on Plant Gene[J];Journal of Anhui Agricultural Sciences;2008-07
4 ZHANG Jin-zhou et al(Department of Animal Science,Henan Institute of Science and Technique,Xinxiang,Henan 453003);Analysis of Population Genetic Distance of Three Carps with RAPD Markers[J];Journal of Anhui Agricultural Sciences;2008-23
5 WANG Dong sheng, YUAN Zhao kai, HUANG Xian ping,XIE Meng zhou, LU Fang guo, YIN Wen xue(Post doctor Station of Hematology, Xiangya Hospital,Central South University, Hu′nan Changsha 410008, China);Relationship Between Syndrome Differentiation of Phlegm & Blood Stasis andPDGF-A mRNA Expression of Peripheral Blood Mononuclear Cell[J];Journal of Anhui Traditional Chinese Medical College;2003-02
6 WANG Xiao-yang, ZHANG Zhen-xiang, XU Yong-jian, HE Min, CHEN Shi-xin, FANG Hui-juan ( Research Laboratory of Respiratory Disease, Tougji Hospital, Tougji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China) ( Institut;Correlation between Expression of Human Telomerase Subunits and Telomerase Activity in Lung Cancer Tissues[J];Chinese Journal of Cancer;2001-06
7 HONG Gui-yun~(1,2),CHEN Hong-quan~1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036; 2.Environment-engineering Department,Anhui Institute of Architecture and Industry,Heifei 230022);Cattle SRY-Specific Sequence Amplification[J];Journal of Anhui Agricultural University;2004-04
8 CHENG AN-chun,WANG Ming-shu,LIU Fei,SONG Yong,YUAN Gui-ping,HAN Xiao-ying,XU Chao,LIAO Yong-hong,ZHOU Wei-guang,WEN Ming,JIA Reng-yong,CHEN Xiao-yueial Animal Disease and Human Health,Avain Disease Research Center,College of Animal Science and Veterinary Medicine,Sichuan Agricultural University,Sichuan,Yaan,625014,China);The Preliminary Application of PCR in Research of Clinical Diagnosis and Mechanisms of Immunity and Pathogeny of Duck Plague Virus (DPV)[J];Chinese Journal of Virology;2004-04
9 Zhang Zhiyi; Lin Shanzhi; Zhang Deqiang; Zhang Qian. College of Biological Science and Biotechnology,Beijing For.Univ., 100083, P. R. China.;Applications of modern molecular biotechnology to genetic improvement in forest trees.[J];Journal of Beijing Forestry University;2002-Z1
10 LU Zhi-qun1,LIU Jing-yuan2,YU Li-jun3(1.Hangzhou First Technician College,Hangzhou 310013,China;2.Gardens Administration of Xuzhou,Xuzhou Jiangsu province 221002,China;3.Fuyang sewage water station,Zhejiang,Hangzhou Zhejiang province 311400,China);An Review on Progress of Forest Genomics[J];Journal of Beijing University of Agriculture;2008-02
China Proceedings of conference Full-text Database 2 Hits
1 YANG Su-li~(1,2) MING Jun~(2,*) LIU Chun~2 MU Ding~2 LI Ming-yang~2 (1 College of Horticulture and Landscape,Southwest University,Chongqing 400715; 2 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Belying 100081);Optimization of SSR-PCR Amplification on Lilium L.[A];[C];2008
2 ;STUDIES ON DETECTION METHODS FOR VSV AND BVDV[A];[C];2005
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HeDe,Tan Xiaofeng,Hu Fangming(The Forestry Ministry Key Laboratory of Economic Forest Breedingand Cultivation,Central Southern Forestry University,Zhuzhou 412006);AFLP molecular marker technique and its aplication on forestry genetics and breeding[J];Journal of Hunan Forestry Scienceand Tehnotogy;1999-04
2 Hu Fangming, Tan Xiaofeng, Li Hong South Central Forestry University, Zhuzhou, Hunan 412006;DNA Extraction from the Endosperm of Torreya Seeds[J];ECONOMIC FOREST RESEACHES;1999-04
3 Su Xiaohua Zhang Qiwen Zheng Xianwu Zhang Xianghua (The Research Institute of Forestry, Chinese Academy of Forestry Beijing 100091) Gui Fu (Foretry Bureau of Beijing Beijing 100029);GENETIC STRUCYURE IN POPULUS USSURIENSIS KOM .CONFIRMED BY RAPD MARKERS[J];SCIENTIA SILVAE SINICAE;1997-06
4 SU Xiao hua 1, LI Jin hua 1, LU Bao min 2 (1.The Research Institute of Forestry,CAF,Beijing 100091;2.The Forestry Station of Seed and Seedling of Beijing,Beijing 100011);Genetic Improvement of Forest and Sustainable Development of Forestry in China for Next Century[J];FOREST RESEARCH;1999-06
5 Yang Minsheng Chen Shaoxiong;Site Preparation for Eucalypti in different Regions[J];ROREST RESEARCH;1997-03
6 ShiJisen (NanjingForestryUniversity Nanjing 2 10 0 37);TheChallengestoForestryBiotechnologyandTreeBreedingin the 2 1stCentury[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-01
7 ShiJisen FuXiangxiang (CollegeofForestryResourcesandEnvironmentNanjingForestryUniversity Nanjing 2 10 0 37);Present Situation and Prospect to In Situ Hybridization in Forest Genetics and Tree-Breeding[J];JOURNAL OF NANJING FORESTRY UNVERSITY;2000-01
8 Xiang Bixia Huang Minren Wang Mingxiu(Key Laboratory of Forest Tree Genetics and Gene Engineering of MFCNanjing Forestry UniversityNanjing210037);THE APPLICATION OF MOLECULAR MARKERS ON GENETICS AND BREEDING OF POPULUS[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1998-02
9 Gan Siming Shi Jisen (Laboratory of Forest Genetics and Gene Engineering Nanjing Forestry University Nanjing 210037)Bai Jiayu Xu Jianmin (Research Institute of Tropical Forestry CAF);RAPD Fingerprints of Clones of Eucalyptus urophylla S. T. Blake and E. tereticornis Smith[J];JOURNAL OF NANJING FORESTRY UNVERSITY;1999-01
10 Yu Zhuo 1 Hu Tingmao 2 (1.Department of Desert Control,Inner Mongolia Forestry College,Hohhot,010019; 2.Faculty of Life Science,Inner Mongolia University,Hohhot,010021);THE APPLICATION OF RFLP MARKER TECHNIQUE IN PLANT BREEDING[J];JOURNAL OF NEIMENGGU FORESTRY COLLEGE;1998-01
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