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《Shaanxi Journal of Traditional Chinese Medicine》 2015-09
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A study of micro-quantitative methods for crystal fiber in herbs from glycyrrhiza uralensis

Yang Zhifeng;Gao Yujuan;Li Jingmei;Shaanxi Institution of Traditional Chinese Medicine;  
Objective:Improve the micro-quantitative methods for crystal fiber in herbs fromglycyrrhiza uralensis.Methods:Based on existing micro-quantitative methods,they replace"Shuifei"sample-making method(the process of which solid powder and liquid are grinded together in order to make suspension or remove contaminants)with Suspending sample-making method,and substitute blood-counting plates for common microscope slides.Besides,we determine sample quantity by linear analysis and optimize the proportion of suspension which is used for plates making as well as the number of sample plates for micro-quantity with orthogonal experiment method.Through inspecting the suspension stability,they could confirm the precise time period of the experiment.Results:The sample fineness should be 100 mesh.We need 10 slides to determine the sample quantity.Suspension:The mix of 60% glycerol and choral hydrate in the ratio of 19∶1.The average number of micro-characteristics of 1mg glycyrrhiza uralensis come from Shenmu is(1498±22)/mg.Conclusion:It is a practical way to use blood-counting plates to increase the ease of operation and accuracy of micro-quantitative method.
【Fund】: 陕西省科技攻关项目(2012K19-03-08)
【CateGory Index】: R284
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHEN Cong-hui,KANG Ting-guo(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China);Research of Correlation between Microscopic Characteristic Constants and Chemical Components of Honeysuckle[J];Journal of Chinese Medicinal Materials;2011-09
【Co-citations】
Chinese Journal Full-text Database 3 Hits
1 LIANG Li1,ZHAO Zhong-zhen2,LI Na1,KANG Ting-guo1(1.Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;2.Hong Kong Baptist University,Kow Loon Tong,Hong Kong);Research on the Correlation between Microscopic Characteristic Constant and Chemical Component of Sophorae Flos[J];Journal of Chinese Medicinal Materials;2013-04
2 LI Da;ZHANG Jing;ZHANG Jian-kui;XU Liang;KANG Ting-guo;Liaoning University of Traditional Chinese Medicine;;Correlation Between Microscopic Characteristic Constants and Chemical Components of Carthami Flos[J];Chinese Journal of Experimental Traditional Medical Formulae;2014-19
3 ZHANG Shan-shan;YUAN Yuan;HUANG Lu-qi;CHEN Ping;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,Chinese Academy of Chinese Medical Sciences;Wuhan Polytechnic University;;Study on characteristics of non-glandular hairs of cultivated Lonicera japonica[J];China Journal of Chinese Materia Medica;2015-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Yuan Dongmin1,Luan Xiaojing2,Ju Qingbo1, Kang Tingguo2 (1.The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China; 2.Liaoning University of Traditional Chinese Medicine, Shenyang 110032,Liaoning,China);A Study on Quantitative Microscopic Method Applied to the Identification of Chinese Material Medica[J];Liaoning Journal of Traditional Chinese Medicine;2006-04
2 YUAN Dong-min1,LIU Yang2,LUAN Xiao-jing2,KANG Ting-guo2(1.The Affiliated Hospital of Liaoning University of TCM,Shenyang 110032,Liaoning,China;2.Liaoning University of TCM,Shenyang 110032,Liaoning,China);The Study on Cortex Phellodendri Chinensis by the Microscopic Quantitative Method[J];Chinese Archives of Traditional Chinese Medicine;2007-05
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