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《Experimental Technology and Management》 2017-05
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Analysis on quality factors of DNA extracted from peripheral blood of Uygur population

Liu Shuhu;Rebiyamu Bake;Bupatima Abudukeremu;Duolikun Maimaitiyusufu;College of Life Sciences and Technology,Xinjiang University;  
Objective:It is to analyze the factors affecting the quality of DNA extracted from the genomic DNA of whole blood frozen samples in Uygur population by different samples and freezing time.Methods:The blood samples at-80 ℃ for 1-4years are extracted by the modified salting-out method,the modified phenol/chloroform method,the KI method and reagent box method.The concentration and purity of DNA are detected by the spectrophotometer and 0.8% agarose gel electrophoresis,and the extracted DNA quality is compared by the single factor variance analysis.Results:The improved KI method is the best to extract DNA,followed by the reagent box method and phenol/chloroform method,and the extraction quality by the saltingout method is the worst.The concentration of DNA extracted from the frozen blood samples for 1-4years is significantly different(F=618.011,P=0.000)and so is its purity(F=27.090,P=0.000).Conclusion:The research is based on economic benefit and easy operation.The quality of DNA can be determined by the improved KI method,and the blood samples frozen(-80 ℃)for more than 3years have significant effect on the quality of extracted DNA.
【Fund】: 国家自然科学基金项目“新疆隔离人群的遗传多样性研究及流行病学资源数据库建立”(31460285);; 新疆维吾尔自治区自然科学基金项目“新疆阿尔克孜族隔离人群减数分裂重组热点遗传定位研究”(2013211A016);; 中国科学院计算生物学重点实验室开放课题(2014KLCB02)
【CateGory Index】: R394
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