The rapid detemination of antioxidant BHT in Food and feed by HPLC
In this paper a method for determining antioxidants BHT in food and animal feed by RP-HPLC on Nova-pak C18(3.9mm i.d.×150mm,60 4μm) using methanol-water-36% acetic acid (85:10:5 volume ratio) as mobile phase was described. The UV detector was set at 280nm. BHT was extracted with 100% methanol. Recoveries were 93.0-100.5% for animal feed and 93.5-117.0% for edible oils . The method was simple, rapid, sensitive and accurate.