Application of Cellulase to Improve the Technological Process of Extracting L-ephedrine
Cheng Julong;Cao Danjun (Department of Basic science, Shenyang Agricultural University)
The traditional process of extracting ephedrine is in the conditions of high temperature (120℃) and high pressure (19.6N/cm~2), it is a wasting energy and polluting environment process. In this project the author extracted ephedrine from ephedra at room temperature and one atmosphere by treating the ephedra residue with cellulase (EC 3,2,1,4β-1, 4-glucose-4-glucanhydrolase). Through a series of experiments. we get a lot of good results mainly as follows: It is feasible that application of cellulase improve the traditional process of extracting ephedrine in high temperature and high pressure. The improved process are extracting the ephedrine from ephedra by water at first and then treat the residue of ephedra with cellulase. the last step is collecting ephe drine from it's water solution with toluene. The optimal conditions for cellulase acting residue after extracting the raw materials by water were at pH 4.5--5.0, 50℃ for 48 h. The inhibition of cellulase by tannin in ephedra is a critical problem in this process, we found a method to eliminate the inhibition of cellulase by tannin, it was adding the anti-tannin agent to the residue of ephedra, and then treated the residue of ephedra by cellulase.
【CateGory Index】： S556 R284.2