Identification and Biological Characterization of Fusarium proliferatum Causing Blossom Blight of Eggplant
HAN Bing;HUANG Yu-fei;ZHANG Xin-yu;FU Jun-fan;LIU Chang-yuan;College of Plant Protection, Shenyang Agricultural University;Institute of Plant Protection, Liaoning Academy of Agricultural Sciences;
In July 2016, a bloom blight was found on the eggplant in greenhouses in Beizhen, Liaoning province. At the early stage of infection, symptoms were found on the petals with water-soaked and pale brown lesions that gradually expanded. With the growth of plants, the pathogen affected the young fruit and pale mold occurred in high humidity, which caused the floral organ being dry. In order to identify the pathogen Fp01 isolated from disease lesions on blossom blight of eggplant, the pathogen pathogenicity was verified with Koch's Rule. The pathogen was preliminarily identified as Fusarium proliferatum according to its morphology, color and growth rate of the colonies, and morphology of macroconidia, chlamydospores, and conidiophores. The fungal rDNA ITS was amplified with ITS4 and ITS5, and sequenced. The sequencing results were accepted by Gen Bank with the accession number of KY237800. Physiological properties of the pathogen were investigated under different culture media,temperature, pH, carbon and nitrogen sources. The results showed that the mycelia of the pathogen could grow rapidly on PSA with optimum temperature of 25℃, pH 7, carbon source maltose and nitrogen source(NH_4)_2SO_4. The mycelia could not survive above 55℃ for 10 min. The optimum temperature for conidial germination was 25℃ with pH 7, carbon source 1% glucan and nitrogen source 1% NH_4Cl. The conidia could not germinate above 54℃ for 10 min.