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《Journal of Applied Clinical Pediatrics》 2009-01
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Hemodynamic Parameter Changes in Pulmonary Arterial Hypertension Treated by Transplantation of Bone Marrow Mesenchymal Stem Cells in Experimental Rats

HE Zhi-xu,WANG Hao-wen,SHANG Feng,YAN Hu,YANG Yan,CUI Dong-bing(Stem Cell Research Center,Guiyang Medical College;Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College,Guiyang 550001,Guizhou Province,China)  
Objective To explore hemodynamic parameter changes in pulmonary arterial hypertension(PAH)treated by transplantation of bone marrow mesenchymal stem cells(MMSCs)in the experimental rats.Methods MMSCs cells were collected from bone marrow of Sprague-Dawleye(SD)rat's femoral and tibial bones,cultured and passaged in vitro,then stained by Hoechst 33342 fluorescence dye stuff.Ninety healthy male SD rats were randomly divided into 3 groups(n=30):normal control group(group N),MMSCs transplanted group(group M),PAH model group(group H).The rats in the two latter groups were given a single subcutaneous crotaline(50 mg/kg)to establish the model of PAH.The rats of group N were injected respectively a single subcutaneous 9 g/L saline water(6 mL/kg).After 21 days,5×109 L-1 MMSCs cultured in 1 mL phosphate-buffered saline were infused into the rats respectively in group M by sublingual vein and 1 mL L-DMEM was given in group H.The indexalso of right ventricle systolic pressure(RVSP),right ventricle hypertrophy index,arterial blood gas analysis and the changes of small pulmonary blood vessel were observed after 28 days.Results The administration of MMSCs 28 days after PAH nearly completely prevented the increase in RVSP with PAH alone [(32.20±2.32)mmHg vs(48.30±1.56)mmHg P0.05],right ventricle hypertrophy indexalso degraded[(38.80±3.24)% vs(45.10±3.43)% P0.05],and relative media thickness raised obviously but the percentage of vessel area(VA%)decreased,results of blood gas analysis such as pH,pa(O2),HCO3-degraded significantly.MMSCs labeled Hoechst 33342 were observed and were permanently planted and differentiated into plentiful collateral circulations in lung by fluorescence microscope.The pulmonary arteries remodeling was attenuated effectively in MMSCs group M.Conclusions These findings indicate that MMSCs might reduce and even reversion the progression of PAH induced by crotaline.And the heart function and hemodynamic parameter can be improved obviously.J Appl Clin Pediatr,2009,24(1):14-17
【Fund】: 国家自然科学基金项目资助(30560159);; 教育部新世纪优秀人才支持计划项目资助(NCET-05-0820);; 贵州省高校自然科学基金资助(黔科教2005-7)
【CateGory Index】: R543
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