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《Journal of Applied Clinical Pediatrics》 2009-01
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Nuclear Factor-κB Activation in Peripheral Blood Mononuclear Cells during Acute Kawasaki Disease

ZOU Zheng1a,XIONG Guo-liang2,DUAN Jun-kai1b,LIU Zhen-qiong2,XU Fei1b,LU Qiao1a(1a.Department of Rheumatology and Immunology;1b.Cardiology Center,Jiangxi Children's Hospital,Nanchang 330006,Jiangxi Province,China;2.Department of Laboratory,Jiangxi Thorax Hospital,Nanchang 330008,Jiangxi Province,China)  
Objective To explore the significance of nuclear factor-κB(NF-κB)activation in peripheral blood mononuclear cells(PBMC)during acute Kawasaki disease(KD).Methods Peripheral blood was collected from children with acute KD(n=30)and healthy age-matched children(n=20).PBMC were cultured in vitro and divided into 3 groups:naturally cultured blank control group,protein kinase C(PKC)activator stimulated phorbol 12-myristate 13-acetate(PMA)group and PMA plus NF-κB inhibitor treated PMA plus pyrrolidine dithiocarbamate(PDTC)group.Percentages of NF-κB activation were detected by immunohistochemistry.Results Under natural culturing,the percentage of cells with activated NF-κB was significantly higher in acute KD blank control group than that in healthy blank control group.The percentage of cells with activated NF-κB was significantly higher in acute KD PMA group than that in acute KD blank group and that in normal control PMA group,respectively(Pa0.01).The percentage of cells with activated NF-κB was significantly lower in acute KD PMA plus PDTC group than that in acute KD PMA group(P0.01).Under natural culturing,the percentage of cells with activated NF-κB in acute KD with coronary artery lesions(CAL)was higher than that in without CAL(P0.01).After PMA stimulation,the above percentage was further higher in acute KD with CAL than that in the disease without CAL(P0.01).There was no correlation between the percentage of NF-κB activation with either serum CRP level or WBC count level in peripheral blood of children with acute KD(Pa0.05).Conclusions NF-κB activation in PBMC during acute KD is markedly increased,which suggests that NF-κB activation plays an important role in the formation of vasulitis and CAL in this disease.NF-κB activation in PBMCs in children with KD is regulated by the PKC signaling pathway and PDTC obviously inhibits the activation of NF-κB.J Appl Clin Pediatr,2009,24(1):35-37
【Fund】: 江西省科技厅社会发展攻关项目(医疗卫生科技研究)资助
【CateGory Index】: R725.4
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