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《Journal of Applied Clinical Pediatrics》 2009-01
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Secretion of Visfatin during 3T3-L1 Preadipocyte Differentiation and Regulative Role of Insulin

CHEN Xiao-hui1,2,ZHANG Min1,2,CHI Xia1,2,QIU Jie2,JI Chen-bo2,ZHANG Chun-mei2,CHEN Rong-hua2,GUO Xi-rong1,2(1.Department of Pediatrics,Nanjing Maternity and Children Health Care Hospital of Nanjing Medical University,Nanjing 210004,Jiangsu Province,China;2.Institute of Pediatrics Medicine,Nanjing Medical University,Nanjing 210029,Jiangsu Province,China)  
Objective To observe the changes of the level of Visfatin protein secreted by 3T3-L1 preadipocytes during adipocyte diffe-rentiation and to explore the regulative role of insulin on Visfatin secretion in adipocytes.Methods 3T3-L1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes with insulin,3-isobutyl-methyl xanthine(MIX),insulin and dexamethasone.Supernatant of cultured cells were collected at various stages of differentiation(0,4,6,8 day).Oil red O dye was used to observe the the process of diffe-rentiation.The regulative role of insulin on Visfatin secretion in adipocytes was observed,100 nmol/L insulin was added into the cultured me-dium on Day 6 or Day 8 of differentiation.The levels of Visfatin in the supernatant of cultured cells were detected by enzyme-linked immunosorbent assay(ELISA).Results There was the gradually rising trend in the levels of Visfatin in the supernatant of cultured cells during the diffe-rentiation of 3T3-L1 preadipocyte.The secretion of Visfatin remained low [(29.0 ± 2.4)μg/L] in preadipocytes,and reached higher level[(48.0 ±3.4)μg/L]in fully differentiated adipocytes.Treatment of 100 nmol/L insulin on adipocytes of Day 6 or Day 8 resulted in increased secretion of Visfatin by the matured adipocytes[(57.9±5.7)μg/L vs(45.7±3.8)μg/L;(61.0±4.2)μg/L vs(48.0±3.4)μg/L Pa0.05].Conclusions There is the relationship between the level of Visfatin secreted by adipocytes and adipocyte differentiation.Insulin may play the role of positive adjustment on the level of Visfatin secreted by adipocytes.J Appl Clin Pediatr,2009,24(1):48-50
【Fund】: 国家自然科学基金项目资助(30571978)
【CateGory Index】: R725.8
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Rui-zhen1,WEN Yu2,XIA Zhi2,MA Xin-yu1,LU Hui-ling2,HU Xiu-fen2,WU Jing2,WANG Xiao-li2,LIN Han-hua2,WANG Hong-wei2(1.Department of Endocrine Diseases,Wuhan Children′s Hospital,Wuhan 430016,Hubei Province,China;2.Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China);Effect of Visfatin on Secretion and Expression of Inflammatory Factors in SW872 Adipocytes[J];Journal of Applied Clinical Pediatrics;2009-19
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1 CHEN Hua-jia,HU Ting(Department of Paediatrics,Sun Yat-Sen University Tungwah Hospital,Dongguan 523110,China);Relationship between insulin resistance and free fatty acids in children with simple obesity[J];Journal of Xinxiang Medical College;2008-03
【Co-citations】
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1 NI Shi-ning,LIU Qian-qi,ZHU Zi-yang,SHEN Jin-pei,XU Jia-chang(Department of Endocrinology,Nanjing Children′s Hospital Affiliated to Nanjing Medical University,Nanjing 210008,Jiangsu Province,China);Relationship between Simple Obesity and Risk Factors of Insulin Resistance Syndrome in Children[J];Journal of Applied Clinical Pediatrics;2009-07
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3 WANG Hong-jie1,ZHAO Ying-zhao2,YANG Qing-yan3(1.Department of Neonatology,Tianjin Children′s Hospital,Tianjin 300074,China;2.Department of Pediatrics,the Health Care Hospital of Women and Children,Xinji 052360,Hebei Province,China;3.Department of Pediatrics,the General Hospital of Tianjin Medical University,Tianjin 300052,China);Expression of Resistin in White Adipose Tissue of Obese Rats[J];Journal of Applied Clinical Pediatrics;2009-08
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1 LI Rui-zhen,XIA Zhi,LU Hui-ling,HU Xiu-fen,WU Jing,WEN Yu,LIN Han-hua,WANG Hong-wei(Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,China);Effect of Visfatin on Glucose Transport in SW872 Adipocyte[J];Journal of Applied Clinical Pediatrics;2008-08
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