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《Journal of Applied Clinical Pediatrics》 2009-02
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Influence of Intra-Amniotic Endotoxin Exposure on Cell Proliferating and Expression of p53 Gene in Lung Tissue of Embryo and Preterm Rat

WANG Wei,YU Xiao,NING Qin,LUO Xiao-ping(Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China)  
Objective To observe the expressions of proliferating cell nuclear antigen(PCNA)and p53 gene in lungs of the embryo and preterm rats exposed to intra-amniotic endotoxin and to explore the relationship between the intrauterine inflammation and the pathogenesis of bronchopulmonary dysplasia(BPD).Methods Scheduled pregnant Sprague Dawley rats were randomly divided into 2 groups:saline group and lipoplysaccharide(LPS)group.LPS(40 μg/L,5 μL)or saline was injected intra-amniotic into the bows on 15tg gestational age day,respectively.The lungs from the 2 groups aged 19 embryonic day(E19)and postnatal day 1,3,5,7(P1,P3,P5,P7)were removed and dissected from the main bronchi for analysis.PCNA protein levels were measured by immunohistochemistry,total RNA was extracted by Trizol reagent from the frozen lung tissues.Lung p53 mRNA levels were measured by semi-quantitative reverse transcription polymerase chain reaction.The results were analyzed by SPSS 12.0 software.Results The contents of PCNA decreased along with the lung development from E19 to P5 in control group and from E19 to P1 in LPS group.However,in LPS group,it was significantly lower than that in the controls from E19 to P3(Pa0.05).The mRNA content change of p53 increased from E19 to P7 in LPS group,and it was significantly higher than that in control groups from E19 to P1(Pa0.05).Conclusions Intrauterine inflammation may be related to the evolution of lung injury and may contribute to the pathogenesis of bronchopulmonary dysplasia by upgrated expressions of p53 gene.
【Fund】: 国家自然科学基金项目资助(30672262 30772358)
【CateGory Index】: R714.5;R722.6
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