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Effects of Polyunsaturated Fatty Acids on Fat Accumulation in Differentiated 3T3-L1 Cells

JIANG Jin1,FAN Chao-nan2,ZHU Hai-yan2,QI Ke-min2 (1.Department of Pediatrics,Anshan Women and Children′s Hospital,Anshan 114014,Liaoning Province,China;2.Clinical Nutrition Center,Beijing Pediatric Research Institute,Beijing Children′s Hospital Affiliated to Capital Medical University,Beijing 100045,China)  
Objective To investigate the effects of n-6 and n-3 polyunsaturated fatty acids(PUFAs) on fat accumulation during adipocyte differentiation in vitro.Methods 3T3-L1 cells were used and incubated respectively with arachidonic acid(AA,C20:4n-6),eicosapentaenoic acid(EPA,C20:5n-3),docosahexaenoic acid(DHA,22:6n-3) or oleic acid(OA,18:1n-9)(0.125 mmol/L) for 12 h after differentiation 5 d,with bovine serum albumin(BSA) as control.Red-oil-O staining was used to observe adipocyte morphological changes.Triglyceride(TG) concentrations in cell and culture medium were measured by glycerophosphate oxidase-phenol aminophenazone(GPO-PAP) and nuclear peroxisome proliferator-activated receptor γ(PPARγ) were determined by enzymelinked immunosorbent assay(ELISA).Results Compared with control group,although addition of AA,EPA or DHA to the medium significantly reduced fat droplets and TG concentrations inside adipocytes(F=3.96,4.34 Pa0.05),no differences were found among the 3 groups.However,addition of OA to the medium did not affect cell TG and fat droplets as compared with BSA.Meanwhile,PPARγ concentration in culture medium was increased,as compared with BSA,when OA,AA,EPA and DHA were added to the medium(F=9.56 P0.01).Further analysis showed those PPARγ concentration in DHA group was lower than that in OA,AA or EPA group(F=6.87 P0.01).Conclusions n-3 and n-6 PUFAs have similar effects in inhibiting fat accumulation in 3T3-L1 cells.However,DHA affects PPARγ expression much less than other fatty acids.
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