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Effects of Dietary Ratio Changes of n-6/n-3 Polyunsaturated Fatty Acids on Expression of Plasma Leptin in Mice

XU Feng,FAN Chao-nan,ZHU Hai-yan,TIAN Chun-yu,QI Ke-min(Clinical Nutrition Center,Beijing Pediatric Research Institute,Beijing Children′s Hospital Affiliated to Capital Medical University,Beijing 100045,China)  
Objective To explore the effects of dietary ratio changes of n-6/n-3 polyunsaturated fatty acids(PUFAs)on expression of plasma leptin and soluble leptin receptor(sOB-R) in mice.Methods Fifty of 3-4 week-old C57BL/6J female mice,which were randomly divided into 5 groups(10 cases in each group),were fed 4 diets with different ratios of n-6/n-3 PUFAs(20:1,10:1,5:1 and 1:1) for 3 months,and n-3 PUFAs deficient diet as controls.The amount of fat in each diet was 6%(weight percentage),and n-6 and n-3 PUFAs were originated from sunflower oil and fish oil,respectively.Plasma leptin and sOB-R concentrations were measured by enzy-me-linked immunosorbent assay(ELISA).SPSS 11.5 software was used to analyze the data.Results There were no significant difference among the weight of 3 groups after different diets of n-6/n-3 PUFAs fed for 3 months.Compared with n-3 PUFA deficient diet,plasma leptin and sOB-R concentrations in mice fed with n-6/n-3 PUFAs(1:1) diet were significantly reduced(F=73.31 P=0;F=7.20 P=0.03),whereas those in mice fed with n-6/n-3 PUFAs(20:1),(10:1) and(5:1) did not change significantly.Conclusions Changes in dietary ratio of n-6/n-3 PUFAs affect the expression of leptin and sOB-R,and higher amount of n-3 PUFAs is needed to reach the inhibitory effects of leptin and sOB-R expression.
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