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Significance of Human Cytomegalovirus Detection in 119 Infantile Cholestasis Syndrome

WANG Yan1,YAN Hui-min2,ZHANG Hui1,YAO Yuan2,HU Yan2,YAN Jing1,XIE Zheng-de1(1.Virology Lab,Beijing Pediatric Research Institute;2.Department of Internal Medicine,Beijing Children′s Hospital Affiliated to Capital Medical University,Beijing 100045,China)  
Objective To investigate the relationship between human cytomegalovirus(CMV) and infantile cholestasis syndrome and the role that serological assay and molecular diagnostic methods play in diagnosis of CMV infection.Methods One hundred and nineteen cases of infantile cholestasis syndrome were enrolled in this study with 71 male and 48 female,whose average age was 2 months,ranging from 1~7 months.Both acute serum and convalescent serum were collected for detection of IgM anti-CMV and CMV DNA.Indirect enzyme linked immunosorbent assay was used to detect IgM anti-CMV in serum.Copies of CMV DNA in serum were detected by real-time fluorescent quantitative polymerase chain reaction(PCR).Results Among 119 cases of infantile cholestasis syndrome,63 cases were positive of CMV,and the positive rate was 52.9%(63/119).Among these positive cases,52 were confirmed by detection of IgM anti-CMV in acute and convalescent se-rum,which including 47 cases with single serum masculine and 5 cases with paired serum negative.Eleven cases(11/63,17.5%) were diagnosed by real-time fluorescent quantitative PCR with negative of IgM anti-CMV in acute and convalescent serum.Of all 119 cases,27 cases were positive of CMV DNA in serum when admitted and 16 cases were positive of IgM anti-CMV and 11 cases were negative of IgM anti-CMV at the same time.Convalescent serum from 15 cases were all negative of CMV DNA.Average copies of CMV DNA in serum were 1.96×106(6.15 ×105-8.76 ×106) copies/L.Conclusions Human CMV infection is common in infantile cholestasis syndrome.Both serum assay and molecular diagnostic method are limited to diagnose human CMV infection.Serum assay and molecular diagnostic met-hod can complement each other.
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