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Changes of the Activity of Nuclear Factor-κB in Neonatal Rat Model of Necrotizing Enterocolitis and Intervention Effect of Intestinal Trefoil Factor on Them

ZHANG Hai-xia,ZHANG Bing-hong,SHI Lei(Department of Pediatrics,the People′s Hospital of Wuhan University,Wuhan 430060,Hubei Province,China)  
Objective To study the effect of intestinal trefoil factor(ITF) on nuclear factor-κB(NF-κB) in neonatal rats intestinal tissues with necrotizing enterocolitis(NEC),and to discuss whether ITF had protective function in NEC,and its role in the mechanism of NEC.Methods Fifty neonatal rats were randomly divided into 5 groups:group A as control group,group B as control plus ITF 0.2 mg group,group C as NEC group,group D as NEC plus normal saline(NS),group E as NEC plus ITF 0.2 mg.NEC models of neonatal rats were established.On the 4th day,all subjects were put to death.The intestinal tissue located at the boundary of ileum and cecum were obtained to observe histological changes and NF-κB level.Results The density of NF-κB(p65) increased significantly in group C and D compared with those in group A,B and E(Pa0.01),and increased significantly in group E compared with those in group A and B(Pa 0.01).Activation of NF-κB(p65) with evident nuclear translocation was found in neonatal rat NEC model.The pathological lesions indicated that intestinal tissue necrosis in varying degrees in group C and D,which were 2-4 points,but obviously lessened in group E,which were 0-2 points.Conclusions Intestinal inflammation is ameliorated after the ITF is injected hypodermically.NF-κB signal pathways might play an important role in the mechanism and signal transduction of NEC.ITF may protect the intestinal tissues of neonatal rat NEC model by suppressing of NF-κB signal transduction pathway.
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