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Effect of Different Courses Hyperbaric Oxygen Treatment on Proliferation and Differentiation of Neural Stem Cells in Hypoxic-Ischemic Brain Damage Rats

WANG Qing-hong,YANG Yu-jia,CHEN Chong-feng,LI Meng,YAO Yue (Department of Neonatology,Xiangya Hospital Affiliated to Central South University,Changsha 410008,Hunan Province,China)  
Objective To explore the effect of different courses hyperbaric oxygen(HBO) promote proliferation and differentiation of neural stem cells(NSCs) in hypoxic-ischemic brain damage(HIBD) newborn rats.Methods Seven-day-old SD rats were subjected to unilateral carotid ligation followed by 2 hours of hypoxia(80 mL/L O2).Difference courses of HBO were administered after 2 hours later(2 ATA,once daily for 7 days a course).At the rats′ age of 35 days,BrdU/Nestin immunofluorescence in the injury subventricular zone(SVZ) and dentate gyrus(DG),BrdU/NSE,BrdU/GFAP immunofluorescence in the injury brain cortex and Nestin protein were detected through Western blot analysis.Meanwhile the other pups were tested with T-maze forced alternation,the radial arm maze and foot-fault.Results 1.The number of BrdU+/Nestin+ cells in SVZ of the HBO treatment groups increased along with the course of HBO,especially in group HBO-3;2.There was no obvious increase of BrdU+/Nestin+ cells in DG of HBO treatment groups;3.The number of BrdU+/NSE+ cells in cortex increased along with the courses of HBO;4.The number of GFAP+ cells in HIBD was higher than that in other groups,but there were few BrdU+/GFAP+ cells in all groups;5.Western blot showed the expression of Nestin protein in group HBO-3 was higher than that in other groups;6.The HBO treatment groups performed better in the behavioral tests along with the courses.Conclusions Multiple courses of HBO treatment may promote neurogenesis of the endogenous NSCs in SVZ and neurons in cortex of perinatal HIBD rats.
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