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《Journal of Applied Clinical Pediatrics》 2009-09
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Effects of Family Allergic History and Dermatophagoides Farina on Expression of Th1/Th2 Cytokine of Cord Blood and Allergic Disorders in Infancy

ZHANG Jian-hua1,SHI Jun2,WU Liang-xia1,WANG Zi-cai1(1.Department of Pediatrics,the Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China;2.Department of Pediatrics,the Second People′s Hospital of Suzhou,Suzhou 215006,Jiangsu Province,China)  
Objective To investigate the effects of family allergic history and dermatophagoides farina on the expressions of Th1/Th2 cytokine of cord blood and allergic disorders in infancy.Methods Ten mil cord blood were obtained from 34 neonates which 17 cases had family allergic history and 17 cases didn′t have.Cord blood mononuclear cells(CBMC) were isolated by gradient centrifugation with Ficoll and were cultured with phytohemagglutinin(PHA) or dermatophagoides farina for 48 hours in vitro.The expressions of interleukin(IL-4) and interferon(IFN-γ) of the culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Two groups were visited with telephone or clinical service every 1 or 2 months in 1 year follow-up survey.Results In no stimulation,the expressions of IL-4 of family allergic history and no family allergic history were(11.35±1.80) ng/L and(11.0±1.50) ng/L,respectively,there was no significant difference.The expressions of IFN-γ were(9.55±1.47) ng/L and(10.19±1.37) ng/L,respectively,there was no significant difference also.In PHA stimulation,the expressions of IL-4 were(43.45±4.57) ng/L and(37.58±3.41) ng/L,respectively,there was significant difference.The expressions of IFN-γ were(72.61±25.40) ng/L and(65.84±29.96) ng/L,respectively,there was no significant diffe-rence.In low density dermatophagoides farina stimulation,the expressions of IL-4 were(40.54±3.64) ng/L and(37.17±2.60) ng/L,respectively,which had significant difference.The expressions of IFN-γ were(35.30±2.73) ng/L and(40.55±1.85) ng/L,respectively,which had significant difference.In high density dermatophagoides farina stimulation,the expressions of IL-4 were(43.50±3.19) ng/L and(39.55±4.13) ng/L,respectively,which had significant difference.The expressions of IFN-γ were(39.40 ±5.21) ng/L and(40.94±2.96) ng/L respectively,which had no significant difference.Allergic diseases were happened in 7 cases of 13 cases with family allergic history and in 2 cases of 15 cases without family allergic history in 1 year follow-up except lost follow-up cases.There were significant difference in 2 groups.Conclusions Th2 cells of cord blood are relative dominant in neonates having family allergic history.Th2 cells relative dominant are more obvious in dermatophagoides farina stimulation.The neonates having family allergic history have a tendency to get allergic diseases in childhood.
【CateGory Index】: R722.1
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