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《Journal of Applied Clinical Pediatrics》 2009-09
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Effects of Family Allergic History and Dermatophagoides Farina on Expression of Th1/Th2 Cytokine of Cord Blood and Allergic Disorders in Infancy

ZHANG Jian-hua1,SHI Jun2,WU Liang-xia1,WANG Zi-cai1(1.Department of Pediatrics,the Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China;2.Department of Pediatrics,the Second People′s Hospital of Suzhou,Suzhou 215006,Jiangsu Province,China)  
Objective To investigate the effects of family allergic history and dermatophagoides farina on the expressions of Th1/Th2 cytokine of cord blood and allergic disorders in infancy.Methods Ten mil cord blood were obtained from 34 neonates which 17 cases had family allergic history and 17 cases didn′t have.Cord blood mononuclear cells(CBMC) were isolated by gradient centrifugation with Ficoll and were cultured with phytohemagglutinin(PHA) or dermatophagoides farina for 48 hours in vitro.The expressions of interleukin(IL-4) and interferon(IFN-γ) of the culture supernatant were detected by enzyme-linked immunosorbent assay(ELISA).Two groups were visited with telephone or clinical service every 1 or 2 months in 1 year follow-up survey.Results In no stimulation,the expressions of IL-4 of family allergic history and no family allergic history were(11.35±1.80) ng/L and(11.0±1.50) ng/L,respectively,there was no significant difference.The expressions of IFN-γ were(9.55±1.47) ng/L and(10.19±1.37) ng/L,respectively,there was no significant difference also.In PHA stimulation,the expressions of IL-4 were(43.45±4.57) ng/L and(37.58±3.41) ng/L,respectively,there was significant difference.The expressions of IFN-γ were(72.61±25.40) ng/L and(65.84±29.96) ng/L,respectively,there was no significant diffe-rence.In low density dermatophagoides farina stimulation,the expressions of IL-4 were(40.54±3.64) ng/L and(37.17±2.60) ng/L,respectively,which had significant difference.The expressions of IFN-γ were(35.30±2.73) ng/L and(40.55±1.85) ng/L,respectively,which had significant difference.In high density dermatophagoides farina stimulation,the expressions of IL-4 were(43.50±3.19) ng/L and(39.55±4.13) ng/L,respectively,which had significant difference.The expressions of IFN-γ were(39.40 ±5.21) ng/L and(40.94±2.96) ng/L respectively,which had no significant difference.Allergic diseases were happened in 7 cases of 13 cases with family allergic history and in 2 cases of 15 cases without family allergic history in 1 year follow-up except lost follow-up cases.There were significant difference in 2 groups.Conclusions Th2 cells of cord blood are relative dominant in neonates having family allergic history.Th2 cells relative dominant are more obvious in dermatophagoides farina stimulation.The neonates having family allergic history have a tendency to get allergic diseases in childhood.
【CateGory Index】: R722.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 LI Zai-ling,ZHOU Wei,SUI Jing,ZHANG Juan,SUN Jin-bo(Department of Pediatrics,Peking University Third Hospital,Beijing 100191,China);Clinical Characteristics of Functional Constipation Relating to Food Allergy in Infants[J];Journal of Applied Clinical Pediatrics;2010-06
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 JIANG Feiling1),SHAN Bao'en2),YAO Min1),WEI Xiaobin1),WU Yan1),DENG Bilan1) 1) Department of Laboratory,People's Hospital of Haikou,Haikou 570208 2) Research Center,the Fourth Affiliated Hospital,Hebei Medical University,Shijiazhuang 050011;Effects of BCG protein extracts on NO production of mouse peritoneal macrophages in vitro[J];Journal of Zhengzhou University(Medical Sciences);2010-04
2 XIE Qing-ling,ZHEN Hong,HU Qiong-yan,TAN Ying,WEN Zhi-hong,LI Liu-qing,LIANG Zhen-hua(Department of Pediatrics,People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi Zhuang Autonomous Region,China);Relationships of Skin Test of Allergens and Serum Dermatophagoides Farina Specific IgE and IgG4 Levels in Children with Allergic Disease[J];Journal of Applied Clinical Pediatrics;2010-04
3 SHI Chang-chun1,WANG Jian-ying1,ZHU Wan-ping2,HUANG Wei-hua2(1.Department of Pediatrics,Jinhua Municipal People′s Hospital,Jinhua 321000,Zhejiang Province,China;2.Zhejiang Provincial Academy of Traditional Chinese Medicine and Pharmacy,Hangzhou 310009,Zhejiang Province,China);Therapeutic Effect of Bergamots Volatile Oil on Asthma[J];Journal of Applied Clinical Pediatrics;2010-04
4 WANG Min,LI Bei,ZHANG Guang-huan(Department of Pediatrics,China Three Gorges University,the First College of Clinical Medical Science,Yichang Central People's Hospital,Yichang 443003,Hubei Province,China);Effect of Melatonin on Transforming Growth Factor-β and Connective Tissue Growth Factor in Asthmatic Mice[J];Journal of Applied Clinical Pediatrics;2010-09
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 CHEN Tong-xin,ZHANG Hui(Department of Pediatrics,Xinhua Hospital,Shanghai Institute for Pediatric Research,Shanghai Jiaotong University School of Medicine,Shanghai 200092,China);Guidelines for Diagnosis and Management of Cow's Milk Protein Allergy in Infants[J];Journal of Applied Clinical Pediatrics;2009-21
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