Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Applied Clinical Pediatrics》 2009-09
Add to Favorite Get Latest Update

Influence of Inhaled Glucocorticosteroid on γ-Glutamylcysteine Synthetase in Inflammatory Cell of Sputum in Children with Asthma

DAI Cong-yu1a,ZHU Yun-fu1b,JIANG Yong-liang1b,LIU Chen-tao2(1a.Department of Pediatrics;1b.Department of Respiratory Medicine,Hunan Provincial Mawangdui Hospital,Changsha 410016,Hunan Province,China;2.Department of Pediatrics,Xiangya Hospital Affiliated to Zhongnan University,Changsha 410001,Hunan Province,China)  
Objective To explore the influence of inhaled glucocorticosteroid on γ-glutamylcysteine synthetase(γ-GCS) in inflammatory cell of sputum in children with asthma.Methods Twenty-two asthmatic children were divided into 2 groups according to treatment.The children who were treated by inhaled budesonide combined with salbutamol were due to group A and the others inhaling salbutamol only were due to group B,the healthy children were acted as healthy control group(group C).The glutation(GSH),total GSH and the activity of γ-GCS in sputum were measured respectively;Expression of γ-GCS in inflammatory cell of sputum were detected by immunohistochemistry;the expression of γ-GCS heavy chain(γ-GCS-h) mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results 1.The total GSH[(1.08±0.14) μmol/L] and oxidized glutathione(GSSG)[(0.37±0.09) μmol/L] were decreased in sputum of group A of post-treatment compared with pre-treatment(Pa0.01),the GSH/GSSG of sputum of group A and group B of pre-treatment(0.28±0.02,0.25±0.08) was decreased compared with group C(Pa0.01).2.The expression of γ-GCS protein [(40.8±3.1)%] and the activity of the γ-GCS(5±2) in inflammatory cell of sputum of group A of post-treatment were decreased compared with pre-treatment(Pa0.01).3.RT-PCR indicated that the expression of γ-GCS mRNA in inflammatory cell of sputum of group A of pre-treatment and group B of pre-treatment(0.65±0.04,0.59±0.03,A valure) were more positive than those of group C(Pa0.01),it was decreased in group A of post-treatment(0.10±0.02,A valure) compared with group A of pre-treatment(0.65±0.04,A valure)(P0.01).4.Linear correlation analysis indicated: there existed strongly positive correlation among total GSH,GSSG and the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in sputum(r=0.781,0.763,0.756;0.899,0.901,0.805 Pa0.01);there existed strongly negative relationship among GSH/GSSG and the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in sputum(r=-0.853,-0.827,-0.836 Pa0.01),there existed strongly positive relationship between the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in inflammatory cell of sputum(r=0.812,0.832,0.845 Pa0.01).Conclusions The glucocorticoid possibly suppressed the expression of γ-GCS-h mRNA,γ-GCS protein and the activity of γ-GCS in sputum of asthmatic children.It is relative that the glucocorticoid decreases the oxidative stress in the inflammatory cell of sputum of asthmatic children.
【CateGory Index】: R725.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 JIANG Shan,SHI Yong-bing,SHI Xiao-song,et al (Department of Nephrology,the Second Hospital affiliated to Suzhou University,Suzhou 215004);The change of periphral CD4~+ and CD25~+ regulatory T cells in patients with asthma[J];Journal of Xinxiang Medical College;2006-05
2 DU Juan1,TAO Wei-hua2,ZUO You1,et al(1.Department of ICU,the Seventh People's Hospital of Shenzhen,Shenzhen 518081,China;2.Department of Respiratory Medicine,the Second Clinical Medicial College of Jinan University,Shenzhen 518081,China);Effect of signal transducer and activators of transcription 5 on airway inflammation of rats with asthma[J];Journal of Xinxiang Medical College;2008-04
3 ZHUN Yun-fu, DAI Ai-guo, HU Rui-cheng, JIAN Yong-lian (Department of Respiratory Medicine, Hunan Institute of Gerontology Hunan Province Geriatric Hospital, Changsha 410001, China);EFFECTS OF Nrf2 ON γ-GLUTAMYLCYSTEINE SYNTHASE IN LUNG OF GUINEA PIGS WITH BRONCHIAL ASTHMA[J];Chinese Journal of Applied Physiology;2006-04
Chinese Journal Full-text Database 10 Hits
1 Pan Daoxiang,Wang Yating,Chen Jiaxin The Department of Pediatric,the First Affiliated Hospital of Anhui Medical University,Hefei 230022;Analysis relative factors of asthma in children and effect with different therapy[J];Anhui Medical Journal;2004-06
2 LI Chuan-jing ,HUANG Qi-ling,XU Hong (Dept of Pediatrics,Suzhou People's Hospital, Suzhou234000);The curative effect of salmeterol and fluticasone propinate inhalation on children asthma[J];Anhui Medical and Pharmaceutical Journal;2005-06
3 Chen Zan People Hospital of Lanxi City, Zhejiang Province(321100);Discussion on the Relationship between Pneumonias Mycoplasma Infection and Children Bronchial Asthma[J];Journal of Zhejiang University of Traditional Chinese Medicine;2009-01
4 Lin Juqing,Huang Chunxiang,Meng Ruanhe Department of Pediatrics,Heji Hospital of Changzhi Medical College;Study on Temperament Characteristic of Children with Asthma[J];Journal of Changzhi Medical College;2009-03
5 Xiao-Ping LIU, Xiao-Yan HU, Bin ZENG, Wei -Jiang QIAN, Xin-gen WANG. Department of Pediatrics, Shenzhen Hospital, Beijing University, Shenzhen, Guangdong 518036, China;Expression of CD40 and CD40 ligand in lymphocytes of children with asthma[J];The Chinese Journal of Contemporary Pediatrics;2005-03
6 WANG Man-Zhi, HE Qing-Nan, YUAN Hong-Xia, LIU Xiao-Liang. Department of Pediatrics, Changsha Central Hospital, Changsha 410004, China;Roles of IL-4, IL-5 and IgE in childhood cough variant asthma[J];Chinese Journal of Contemporary Pediatrics;2006-05
7 PENG Shu-Mei, SUN Ping, ZENG Jing, DENG Xue-Mei. Guangdong Province Maternal and Child Health Care Hospital, Guangzhou 510010, China;Cardiac function of children with bronchial asthma[J];Chinese Journal of Contemporary Pediatrics;2006-05
8 XIANG Li, SHEN Kun-Ling, ZHANG Qi, XIONG Zhen-Yi. Center of Asthma, Bejing Children′s Hospital Affiliated to Capital University of Medical Science, Beijing 100045, China;Atopy spectrum and its relationship with clinical characteristics in asthmatic children under 4 years of age[J];Chinese Journal of Contemporary Pediatrics;2006-06
9 LIU Xiao-Mei, CHE Shu-Yu, LIU Xiang-Rong, LIN Rong-Jun. Department of Pediatrics, Affiliated Hospital, Qingdao University School of Medicine, Qingdao, Shandong 266011, China;Values of impulse oscillometry for pulmonary function and adhesion molecules in childhood asthma[J];Chinese Journal of Contemporary Pediatrics;2007-05
10 YING Yan-Feng,HU Ye,SHAN Xiao-Yun,DU Juan,TU Ping-Guang.Jinhua College of Professional and Technology,Jinhua,Zhejiang 321007,China;Changes of prostaglandin D2 receptor on T cells in peripheral blood of children with asthma[J];Chinese Journal of Contemporary Pediatrics;2009-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Jin,WU Hao,FANG Ping,ZHOU Chao(Department of Respiratory Disease,The People's Hospital of Tongling City,Anhui 244000,China);The survey of the common inhaied allergen in the patients with asthma in Tongling area of China[J];Chinese Journal of Clinical Healthcare;2006-04
2 ZHANG Jian-hua~(1),ZHANG Xue-lan~(2),JI Zheng-hua~(2),SHENG Jin-Yun~(2)(1.Department of Pediatrics,the Sixth People′s Hospital Affiliated to Shanghai Jiaotong University,Shanghai 200233,China;2.Suzhou Children′s Hospital Affiliated to Suzhou University,Suzhou 215003,China);Study on Effects of AngiotensinⅡ on Inflammation in Asthmatic Children[J];Journal of Applied Clinical Pediatrics;2006-04
3 FANG Sheng~1,YE Zi~2(1.Taizhou Hospital of Zhejiang Province;2.the First Hospital of Linhai,Linhai 317000,China);Effect of Asarone Injection on Airway Inflammation Factor in Asthmatic Children[J];Journal of Applied Clinical Pediatrics;2006-04
4 CUI Ai-hua~1,FENG Yi-zhen~2,SUN Xiao-jing~1,LIANG Jun~1,ZHANG Ming-xia~1 (1.Liaocheng People′s Hospital, Liaocheng 252000,China;2. Shandong Provincial Hospital of Shandong University, Jinan 250001,China);Correlation between Serum Interleukin -4,12 and Cellular Immunity in Children with Asthma[J];Journal of Applied Clinical Pediatrics;2006-16
5 CHEN Yuan-dong~1,LU Hong-yan~2,FAN Shan~1,JIANG Ru-gang~1(1.Department of Preventive Medicine,Xianning College,Xianning 437100,China;2.Department of Pediatrics,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Effects of Zinc Deficiency on Th1/Th2 Cytokine in Asthmatic Rats[J];Journal of Applied Clinical Pediatrics;2006-21
6 WANG Hong-wei1,QU Shu-qiang1,GAO Jun2,ZHANG Feng-xia1(1.Department of Pediatrics,the Fifth Affiliated Hospital of Haerbin Medical University,Daqing 163316,China;2.Department of Pedia-trics,the Second Affiliated Hospital of Haerbin Medical University,Haerbin 150001,China);Significance of Determining Interleukin-17,Matrix Metalloproteinases-9 and Immunoglobulin E in Serum of Asthmatic Children[J];Journal of Applied Clinical Pediatrics;2007-04
7 QIAN Li-juan1,YANG Li1,YU Hai-guo2a,ZHAO De-yu2b(1.School of Clinical Medicine,the Southeast University,Nanjing 210009,China;2a.Department of Immunology;2b.Department of Pulmonary,Children′s Hospital Affiliated to Nanjing Medical University,Nanjing 210008,China);Expressions of GATA-3 and Th2 Cytokines in Asthmatic Mice and Their Significances[J];Journal of Applied Clinical Pediatrics;2007-04
8 WANG Lei1a,CAO Gao-zhong2,YE Le-ping1b,FANG Li1b(1a.Department of Pharmacy;1b.Department of Respiratory Medicine,the Second Hospital,Wenzhou 325027,China;2.Department of Pharmacy,the First Hospital Affiliated to Wenzhou Medical College,Wenzhou 325000,China);Modulation of Lentinan on Airway Inflammation and Helper T Lymphocyte Cell 1/Helper T Lymphocyte Cell 2 Imbalance in Rats′ Model of Asthma[J];Journal of Applied Clinical Pediatrics;2007-21
9 GUO Hai-ying,CHEN Qin,ZhOU Xin(Department of Respiratory Medicine,the First People's Hospital of Shanghai JiaoTong University,Shanghai 200080,China);Clinical analysis of 33 cases of cough variant asthma[J];Journal of Xinxiang Medical College;2006-01
10 YUAN Xiao-mei,GUO Yue-peng(Department of Respiratory,the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China);Correlation of transforming growth factorβ1 and asthma and advances on treatment of ligustrazine on asthma[J];Journal of Xinxiang Medical College;2006-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved