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《Journal of Applied Clinical Pediatrics》 2009-09
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Influence of Inhaled Glucocorticosteroid on γ-Glutamylcysteine Synthetase in Inflammatory Cell of Sputum in Children with Asthma

DAI Cong-yu1a,ZHU Yun-fu1b,JIANG Yong-liang1b,LIU Chen-tao2(1a.Department of Pediatrics;1b.Department of Respiratory Medicine,Hunan Provincial Mawangdui Hospital,Changsha 410016,Hunan Province,China;2.Department of Pediatrics,Xiangya Hospital Affiliated to Zhongnan University,Changsha 410001,Hunan Province,China)  
Objective To explore the influence of inhaled glucocorticosteroid on γ-glutamylcysteine synthetase(γ-GCS) in inflammatory cell of sputum in children with asthma.Methods Twenty-two asthmatic children were divided into 2 groups according to treatment.The children who were treated by inhaled budesonide combined with salbutamol were due to group A and the others inhaling salbutamol only were due to group B,the healthy children were acted as healthy control group(group C).The glutation(GSH),total GSH and the activity of γ-GCS in sputum were measured respectively;Expression of γ-GCS in inflammatory cell of sputum were detected by immunohistochemistry;the expression of γ-GCS heavy chain(γ-GCS-h) mRNA were detected by reverse transcriptase-polymerase chain reaction(RT-PCR).Results 1.The total GSH[(1.08±0.14) μmol/L] and oxidized glutathione(GSSG)[(0.37±0.09) μmol/L] were decreased in sputum of group A of post-treatment compared with pre-treatment(Pa0.01),the GSH/GSSG of sputum of group A and group B of pre-treatment(0.28±0.02,0.25±0.08) was decreased compared with group C(Pa0.01).2.The expression of γ-GCS protein [(40.8±3.1)%] and the activity of the γ-GCS(5±2) in inflammatory cell of sputum of group A of post-treatment were decreased compared with pre-treatment(Pa0.01).3.RT-PCR indicated that the expression of γ-GCS mRNA in inflammatory cell of sputum of group A of pre-treatment and group B of pre-treatment(0.65±0.04,0.59±0.03,A valure) were more positive than those of group C(Pa0.01),it was decreased in group A of post-treatment(0.10±0.02,A valure) compared with group A of pre-treatment(0.65±0.04,A valure)(P0.01).4.Linear correlation analysis indicated: there existed strongly positive correlation among total GSH,GSSG and the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in sputum(r=0.781,0.763,0.756;0.899,0.901,0.805 Pa0.01);there existed strongly negative relationship among GSH/GSSG and the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in sputum(r=-0.853,-0.827,-0.836 Pa0.01),there existed strongly positive relationship between the expression of γ-GCS-h mRNA,γ-GCS protein,the activity of γ-GCS in inflammatory cell of sputum(r=0.812,0.832,0.845 Pa0.01).Conclusions The glucocorticoid possibly suppressed the expression of γ-GCS-h mRNA,γ-GCS protein and the activity of γ-GCS in sputum of asthmatic children.It is relative that the glucocorticoid decreases the oxidative stress in the inflammatory cell of sputum of asthmatic children.
【CateGory Index】: R725.6
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