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《Journal of Applied Clinical Pediatrics》 2009-15
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Study on Directional Differentiation of Bone Marrow Mesenchymal Stem Cells into Adipose Cells and Neuron-Like Cells in Rats

WANG Hao-wen1,HE Zhi-xu2,WANG Zhi-hua2,LIU Jun-feng2(1.Department of Neonatology,Chizhou People's Hospital,the Affiliated Chizhou Hospital of Wannan Medical College,Chizhou 247100,Anhui Province,China; 2.Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College,Stem Cell Research Center of Guiyang Medical College,Guiyang 550004,Guizhou Province,China)  
Objective To explore the cultivation of rat bone marrow mesenchymal stem cells(MSCs) and the potency of differentiating into adipose cells and neuron-like cells in vitro.Methods MSCs were obtained from bone marrow of SD rat's femoral and tibial bones,cultured and propagated in vitro,then purified by adhesive-screening methods.The morphologic changes of MSCs were observed by inverted light microscope.The expressions of CD71,CD29,CD34 and CD45 in MSCs were detected and identified by flow cytometry.The 5th passage MSCs were induced to differentiate into adipose cells in induction medium containing desamethasone and insulin.Then differentiated cells were identificated by oil red O stain.After treatment with basic fibroblast factor(bFGF),dimethyl sulfoxide(DMSO) and butyl hydroxy anisole(BHA),MSCs could be differentiated into cells with typical neuronal morphology.The expressions of neuron specifice nolase(NSE),Nestin and glialfibriliary acidic protein(GFAP)were detected by immunocytochemistry.Results The results of inverted light microscope showed that the cultured MSCs appeared no difference,generally were spindle-shaped or star-shaped epidermal-liked cells. The cellular volume increased and fibroblast-like cells were increased after serial subcultivation.The 3th passage cells were detected by flow cytometry and the results turned out to be positive expressions of CD29,CD71. After induction,they were confirmed to be adipose cells by oil red O stain and neuron-like cells by immunocytochemistry staining,expressed NSE,Nestin,none of them expressed GFAP.Conclusions In vitro,MSCs can be induced into adipose cells and neuron-like cells,which shows that MSCs have multipotency and are one of the best seed cells in the tissue engineering.
【Fund】: 国家自然科学基金项目资助(30560159);; 教育部“新世纪优秀人才支持计划”(NCET-05-0820);; 贵州省科技攻关项目基金项目资助[黔科字(2008)3026号]
【CateGory Index】: R329
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