Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Applied Clinical Pediatrics》 2009-15
Add to Favorite Get Latest Update

Study on Directional Differentiation of Bone Marrow Mesenchymal Stem Cells into Adipose Cells and Neuron-Like Cells in Rats

WANG Hao-wen1,HE Zhi-xu2,WANG Zhi-hua2,LIU Jun-feng2(1.Department of Neonatology,Chizhou People's Hospital,the Affiliated Chizhou Hospital of Wannan Medical College,Chizhou 247100,Anhui Province,China; 2.Department of Pediatrics,the Affiliated Hospital of Guiyang Medical College,Stem Cell Research Center of Guiyang Medical College,Guiyang 550004,Guizhou Province,China)  
Objective To explore the cultivation of rat bone marrow mesenchymal stem cells(MSCs) and the potency of differentiating into adipose cells and neuron-like cells in vitro.Methods MSCs were obtained from bone marrow of SD rat's femoral and tibial bones,cultured and propagated in vitro,then purified by adhesive-screening methods.The morphologic changes of MSCs were observed by inverted light microscope.The expressions of CD71,CD29,CD34 and CD45 in MSCs were detected and identified by flow cytometry.The 5th passage MSCs were induced to differentiate into adipose cells in induction medium containing desamethasone and insulin.Then differentiated cells were identificated by oil red O stain.After treatment with basic fibroblast factor(bFGF),dimethyl sulfoxide(DMSO) and butyl hydroxy anisole(BHA),MSCs could be differentiated into cells with typical neuronal morphology.The expressions of neuron specifice nolase(NSE),Nestin and glialfibriliary acidic protein(GFAP)were detected by immunocytochemistry.Results The results of inverted light microscope showed that the cultured MSCs appeared no difference,generally were spindle-shaped or star-shaped epidermal-liked cells. The cellular volume increased and fibroblast-like cells were increased after serial subcultivation.The 3th passage cells were detected by flow cytometry and the results turned out to be positive expressions of CD29,CD71. After induction,they were confirmed to be adipose cells by oil red O stain and neuron-like cells by immunocytochemistry staining,expressed NSE,Nestin,none of them expressed GFAP.Conclusions In vitro,MSCs can be induced into adipose cells and neuron-like cells,which shows that MSCs have multipotency and are one of the best seed cells in the tissue engineering.
【Fund】: 国家自然科学基金项目资助(30560159);; 教育部“新世纪优秀人才支持计划”(NCET-05-0820);; 贵州省科技攻关项目基金项目资助[黔科字(2008)3026号]
【CateGory Index】: R329
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 4 Hits
1 CHEN Bingyao,HOU Shuxun,ZHAO Min,et al.Department of Orthopaedics,the First Accessory Hospital of the General Hospital of PLA,Beijing 100037,China;Maintaining culture following neuronal induction of rat marrow derived mesenchymal stem cells[J];Chinese Journal of Bone Tumor and Bone Disease;2007-06
2 WANG Yan,CHEN Yan-ling,BEN Xiao-ming,YU Wen-liang,ZHOU Xiao-yu,MIAO Deng-shun(Newborn Medical Center,Nanjing Children′s Hospital of Nanjing Medical University,Nanjing 210008,China);Isolation in Vitro and Identification of Non-Adherent Bone Marrow Mesenchymal Stem Cells of Mouse[J];Journal of Applied Clinical Pediatrics;2008-07
3 GUAN Ya-fei1, LU Chao1, CHEN JI-qing1, YUAN Chuan-shun2, ZHOU Guo-ping1, ZHOU Yan1, HUANG Song-ming1, GUO Xi-rong1, CHEN Rong-hua1 (1.Department of Pediatrics,the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;2. Department of Pe-diatrics,Qishuyan Region Hospital, Changzhou 213011,China);Changes of Calpain-10 Gene Transcription in Bone Marrow Stem Cells Treated by Serum Deprivation in Rats[J];Journal of Applied Clinical Pediatrics;2007-03
4 Qi XY Zhang J Li YX Li SR Meng CL Dang Y Wang TH. Department of Cardiology,Hebei Provincial People's Hospital,Shijiazhuang 050051,Hebei Province.China;Occurrence of ventricular tachycardia and the expression of connexin 43 after autologous mesenchymal stem cell transplantation[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-50
Chinese Journal Full-text Database 5 Hits
1 Yu Peying, Zheng Zhixiu, Cai Zhiling, et al Department of Pediatrics, Seventeen Ye Hospital, Maanshan 243000;Expression of Smac gene in bone mesenchymal stem cells cultured by glutamate induced in rats and Its Significance[J];Anhui Medical Journal;2008-05
2 YU Pei-ying , ZHENG Zhi-xiu, CAI Zhi-ling, ZANG Jing-hong, WU Ying-rao, YAO Xiao-fei, JIANG Li, CHEN Ji-qing,LU Chao, GUAN Ya-fei. Department of Pediatrics, 17th Metallargy Hospital,Ma'anshan 243000, China;The role of Glutamate in inducing damage of bone marrow mesenchymal stem cells in rats[J];Chinese Medical of Factory and Mine;2008-06
3 Wang Yan1, Chen Yan-ling2, Ben Xiao-ming2, Miao Deng-shun2 1Neonatal Department, Tai’an Central Hospital, Tai’an 271000, Shandong Province, China; 2Neonate Medical Center, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, Nanjing 210008, Jiangsu Province, China;Effects of epithelial growth factor on colony-forming efficiency of non-adherent bone marrow mesenchymal stem cells[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-23
4 Dang Yi, Qi Xiao-yong, Meng Cun-liang, Li Shu-ren, Li Ying-xiao, Liu Hui-liang, Wang Tian-hongHeart Center, Hebei Provincial People’s Hospital, Shijiazhuang 050051, Hebei Province, China;Electrophysiological characteristics of bone marrow stem cells following in vitro induction[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-27
5 Liu Hui1, 2, Wang Jing1, Yan Zhi-feng1, Peng Hong-mei1, Zhou Hong-hui1, Li Jie1, Li Ya-li11Department of Gynaecology and Obstetrics, General Hospital of Chinese PLA, Beijing 100850, China;2Research Room of Stem Cells and Regeneration Medicine, Institute of Field Battle and Blood Transfusion, Academy of Military Medical Sciences of Chinese PLA, Beijing 100850, China;Differentiation of full-term amniotic fluid stem cells into neurons under the induction of serum deprivation and beta-mercaptoethanol[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-19
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 HUANG Yue,XU Yuming,SONG Bo,et alDepartment of Neurology,Henan Provincial People's Hospital,Zhengzhou 450003,China;Mechanism on Marrow Stromal Cells Transplanting for Treatment of Cerebral Ischemia-Reperfusion Injury in Rats[J];Journal of Medical Forum;2006-17
2 Han Zongchao,Zhang Suming,Zhang Min et al Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030;The DNA Damage and Repair and Apoptosis in PC12 Cells Induced by Anoxia and Serum Deprivation[J];Acta Universitatis Medictnae Tangji;2001-02
3 Pan Dengji, Zhang Min, Yang Mingshan Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030;Changes of Cell Cycle and Cell Death in PC12 Cells in Serum-deprivation Medium[J];Acta Universitatis Medictnae Tangji;2004-05
4 Feng Cao,Li Li Niu,Peng Zhang,Lin Chen,Guo Liang Jia,Xue Tao PeiFeng Cao,Li Li Niu,Peng Zhang,Lin Chen,Xue Tao Pei,Beijing Institute of Blood Transfusion Medicine,Beijing 100850,ChinaFeng Cao,Guo Liang Jia,Department of Cardiology,Xijing Hospital,Fourth Military Medical University of Chinese PLA,Xi'an 710032,Shaanxi Province,China;Effect of transplantation of exogenous bone marrow mesenchymal stem cells for repairing chronic myocardial infarction on left ventricular function[J];Chinese Journal of Clinical Rehabilitation;2004-24
5 WU Sheng-hua, LU Chao, DONG Ling (Department of Pediatrics, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China);Calpain 10 mediates lipoxin A_4-induced apoptosis in rat renal interstitial fibroblasts[J];Chinese Journal of Pathophysiology;2006-01
6 Ma Guo-tao, Ren Hua, Zhao Ming, Zhang Chao-ji, Pei MingDepartment of Cardiac Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China;A control observation on transplantation of bone marrow-derived mesenchymal stem cells and myogentic cells for improving cardiac function in rabbits with ischemic heart disease[J];Chinese Journal of Clinical Rehabilitation;2006-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved