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《Journal of Applied Clinical Pediatrics》 2009-15
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Relationship between Apoptosis of Bone Marrow Cells and Tumor Necrosis Factor-α,Fas/FasL Expression in Children with Aplastic Anemia

ZHAO Xiao-qing,MA Fu-tian,ZHANG Bao-xi,WU Xiao-li(Department of Pediatrics,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei Province,China)  
Objective To investigate the relationship between apoptosis of bone marrow cells and tumor necrosis factor-α(TNF-α),Fas/FasL expression in children with aplastic anemia.Methods The concentration of TNF-α in supernatant of cultured bone marrow mononuclear cell(BMMC) was measured by enzyme linked immunosorbent assay.The expression of Fas/FasL,apoptosis ratio of bone marrow CD34+ cell were evaluated by flow cytometry.Results The concentration of TNF-α in supernatant of cultured BMMC in AA group[SAA (27.9±8.20) ng/L;CAA (23.6±6.78) ng/L] increased significantly compared with control group(t=3.432 P0.01).The expression of Fas/FasL in SAA and CAA group were (25.88±5.75)%,(5.87±0.56)% and (23.51±3.13)%,(6.73±0.98)%.Compared with control group,the expression of Fas was significantly different (t=3.446 P0.01),but the expression of FasL was no different (t=1.083 P0.05).Apoptosis ratio of CD34+ cell in SAA group[(24.6±9.56)%] and CAA group[(20.9±7.32)%] significantly increased compared with control group(t=3.492,3.458 Pa0.01),but there were no difference between SAA and CAA group in terms of the concentration of TNF-α,the expression of Fas/FasL and apoptosis ratio (Pa0.05).The concentration of TNF-α was positively correlated with the expression of CD34+ Fas+ in all types AA children(r=0.542 P0.05),and apoptosis ratio of bone marrow CD34+ cell was positively correlated with the expression of CD34+ Fas+ in all types AA children too(r=0.602 P0.05).However,there was no correlation between the concentration of TNF-α and apoptosis ratio of bone marrow CD34+ cell(r=0.086 P0.05).Conclusions TNF-α and Fas/FasL system can be involved in inducing CD34+ cell apoptosis.This may contribute to understanding the decreased number of stem cells and bone marrow failure.
【CateGory Index】: R725.5
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