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《Journal of Applied Clinical Pediatrics》 2009-18
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Influence of Heme Oxygenase-1 Inhibitor Protoporphyrin Ⅸ Zinc on Hyperoxic Lung Injury in Preterm Rats

QUAN Yu-feng1,ZHENG Ming-ci2,ZHANG Hua1,ZHANG Pei-lin1,ZHANG Hong1(1.Department of Neonatology;2.Perinatology of Laboratory,the Affiliated Hospital of Guilin Medical College,Guilin 541001,Guangxi Zhuang Autonomous Region,China)  
Objective To explore the effect of protoporphyrin Ⅸ zinc(Znpp) on hyperoxic lung injury in preterm rats.Methods Three-day-old preterm SD rats were randomly assigned to room air control group(group Ⅰ)hyperoxia control group(oxygen≥900 mL/L)(group Ⅱ),room air plus Znpp group(group Ⅲ),hyperoxia plus Znpp group(group Ⅳ).Group Ⅲ and group Ⅳ were injected intraperitoneally with ZnPP 45 μmol/kg each day.After the third day and the 7th day of exposure,the activity of heme oxygenase-1(HO-1) and the percent of carboxyhemoglobin(HbCO) in the lungs,the lung wet weight /dry weight ratio(W/D),tumor necrosis factor-α(TNF-α),total protein and malondialdehyde(MDA) in bronchoalveolar lavage fluid were determined and lung histophathological changes were examined in all groups.Results On the third day,compared with group Ⅰ the activity of HO-1 and the percent of HbCO in the lungs,W/D,TNF-α,total protein and MDA,all greatly rised in group Ⅱ(Pa0.05,0.01,respectively),and the activity of HO-1 and the percent of HbCO in the lungs,W/D,TNF-α,MDA in group Ⅳ significantly dropped compared with those in group Ⅱ(Pa0.01,0.05,respectively);On the 7th day,the activity of HO-1 and the percent of HbCO in the lungs,TNF-α,total protein,MDA in group Ⅱ all went up markedly compared with those in group Ⅰ(Pa0.01),and the activity of HO-1,the percent of HbCO in the lungs,TNF-α,total protein,MDA in group Ⅳ all significantly reduced compared with those in group Ⅱ(Pa0.01,0.05,respectively).Group Ⅱ showed acute lung injury characterized by the presence of hyperaemia,red cell extravasation,and inflammatory infiltration after 3 days,the pulmonary pathologic changes alleviated in group Ⅳcompared with that in group Ⅱ.After 7 days of exposure,Group Ⅱ showed subacute inflammatory response,pulmonary fibrosis,alveolar stunting,while the changes were relieved in group Ⅳ compared with that in group Ⅱ.Conclusions HO-1 could be associated with the hyperoxia-induced lung injury of premature rats.Znpp may play a protective role against hyperoxia-induced pulmonary damage by inhibiting the activity of HO-1.
【Fund】: 广西科技厅科技攻关基金项目资助(0472002-35)
【CateGory Index】: R722.6
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