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《Journal of Applied Clinical Pediatrics》 2012-23
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Change of Tumor Necrosis Factor-α Expression in Mice with Obesity-Related Glomerulopathy

JIANG Hong-kun1,LI Lei2,JIANG Hong1,LI Ling3,NIU Zhi-bin2(1.Department of Pediatrics,the First Affiliated Hospital of China Medical University,Shenyang 110001,Liaoning Province,China;2.Department of Surgery,Shengjing Hospital Affiliated to China Medical University,Shenyang 110003,Liaoning Province,China;3.Institute of Medical Genetics,School of Life Science & Key Laboratory for Bio-resources and Eco-environment of the Ministry of Education,Sichuan University,Chengdu 610064,Sichuan Province,China)  
Objective To detect the serum TNF-α and glomerular TNF-α mRNA and protein expressions in obesity-related glome-rulopathy(ORG) mice,in order to explore the pathogenesis of ORG. Methods Clean grade 35 d age C57BL/6 male mice were randomly divided into obesity group(20 cases) and control group(20 cases).The obesity group was fed with a high-fat high-energy diet,while the control group was fed with a normal diet.Eight weeks later,the 2 groups were assayed with enzyme linked immunosorbent assay(ELISA) for urinary microalbumin series proteins including urinary albumin(Alb),transferrin(TRF),retinol binding protein(RBP),and β2-microglobulin(β2-MG).Blood samples were also assayed with ELISA for serum TNF-α.Kidney tissue was fixed,sectioned and stained,and examined under both light and electron microscopy for histopathological changes.Renal tissue RNA was extracted and real-time quantitative polymerase chain reaction(RT-PCR) was carried out to measure the expression of TNF-α mRNA.TNF-α protein expression was assayed with Western blot.The difference between the 2 groups was assessed with SPSS 13.0 software. Results Compared with the control group,the obesity group had significantly higher urinary Alb,TRF,RBP and β2-MG and serum TNF-α levels(Pa 0.01).TNF-α mRNA and protein expressions of the renal tissue of the obesity group were significantly higher(Pa0.01).Histopathological examination found that the obesity group had glomerular hypertrophy.Electron microscope revealed epithelial cell foot process fusion,more lipid droplets in the cytoplasm of renal tubular epithelial cells,thickening of basement membrane,loss of the 3-layer structure,with sections insert into the segment mesangial. Conclusions ORG mice appear abnormal urine microalbumin;expression of TNF-α in serum and renal tissue abnormalities may play an important role in the occurrence and development of ORG.
【Fund】: 国家自然科学基金(30571867 81072899);; 辽宁省科技厅基金(2009225010-207)
【CateGory Index】: R589.2;R692.6
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