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《Journal of Applied Clinical Pediatrics》 2012-23
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Expression of SOX10 mRNA in Bowel of Children with Hirschsprung′s Disease

LI Xin-ning,ZENG Tian,MO Dan,SHI Qun-feng,LUO Shu-you,SU Nai-wei (Department of Pediatric Surgery,Guangxi Children′s Hospital,Nanning 530003,Guangxi Zhuang Autonomous Region,China)  
Objective To investigate the expression of SOX10 mRNA in bowels of Hirschsprung′s disease(HD) patients,and to further explore the molecular mechanism of HD. Methods The specimens were collected from 50 HD patients,and the negative samples from 50 patients without HD were used as controls.Smooth muscle tissues were isolated from spastic segment,transition segment and distending segment,respectively.RNA of each segment was extracted,then the targeting gene and housekeeping gene(β-actin) were amplified by means of reverse transcriptions polymerase chain reaction,and the expression level of SOX10 mRNA was subjected to a comparative examination in the trial and control groups.The data were analyzed by using SPSS 17.0 statistical software. Results SOX10 mRNA in spastic segment(0.186 19±0.007 84) showed low expression,which displayed statistical difference from that in transitional segment(0.314 71±0.016 57),distending segment(0.506 94±0.006 31) and control group(0.594 35±0.006 29)(F=16 384.220,P=0.000).In contrast,a comparative high expression in distending segment was observed.However,SOX10 mRNA level in transitional segment and distension segment was significantly lower than that in control group(F=8 666.046,P=0.000). Conclusions The abnormal expression in bowel segments of HD patients infers that SOX10 is necessary to maintain the function of enteric nervous system.The insufficient expression of SOX10 mRNA may cause the relevant bowel dysfunctions,including intestinal stenosis and spasm.
【Fund】: 广西科技厅自然基金(桂科自0991181)
【CateGory Index】: R726.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 GAO He-yun,WEI Ming-fa,WU Xiao-juan,XIANG Lei(Department of Pediatric Surgery,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China);Identification and Establishment of Sprague-Dawlay Rat′s Model with Experimental Congenital Megacolon[J];Journal of Applied Clinical Pediatrics;2010-23
2 WU Xiao-juan,FENG Jie-xiong(Department of Pediatric Surgery,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China);Diagnosis and Treatment for Hirschsprung's Disease[J];Journal of Applied Clinical Pediatrics;2011-11
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 SHI Qun-feng,LI Xin-ning.Department of Pediatric Surgery,Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region,Nanning 530003,China;Complications and their prevention and treatment of laparoscopic-assisted Soave radical operation in the treatment of congenital megacolon in child[J];Journal of Laparoscopic Surgery;2013-03
2 LIU Yuan-mei(Department of Pediatric Surgery,the Affiliated Hospital of Zunyi Medical College,Zunyi 563003,Guizhou Province,China);Clinical Application of Laparoscope Assisted Pull-Out Operation for Long-Segment Type Hirschsprung′s Disease[J];Journal of Applied Clinical Pediatrics;2011-23
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 HOU Yu,YANG Ye,ZHAO Xin,TONG Hai-xia,WANG Bao-xi(Department of Pediatrics,Tangdu Hospital of the Fourth Military Medical University,Xi′an 710038,China);Expression of Glial Cell Derived Neurotrophic Factor in Hirschsprung's Disease[J];Journal of Applied Clinical Pediatrics;2008-07
2 WANG Guo, WENG Yizhen, WEI Mingfa, SUN Xiaoyi. Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Long-term follow-up of Hirschsprung′s disease patients treated by heart-shaped anastomosis operation[J];Chiese Journal of Surgery;2002-05
3 FENG Jie-xiong, SHI Hui-fen, WANG Guo, HAN Jie,GUO Xian-e,WEI Ming-fa,YI Bin,SUN Xiao-yi. Department of Pediatric Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Analysis of the acetylcholinestase activity in rectal suction biopsy of 1008 children with constipation[J];Chinese Journal of Pediatric Surgery;2004-06
4 JIN Xian-qing~* XU Ji-rong CHEN Xiao-zhang Department of Surgery,Children's Hospital,Chongqing University of Medical Sciences,Chongqing 400014,China;A preliminary study on the treatment of experimental aganglionosis by mesenchymal stem cells transplantation into colon in vivo[J];Chinese Journal of Pediatric Surgery;2007-09
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