Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Applied Clinical Pediatrics》 2012-24
Add to Favorite Get Latest Update

Protective Effect of Glutamine on Lung Injury Induced by Hyperoxia in Neonatal Rats

ZHANG Hai-hong,LIU Xiu-xiang,ZHAO Guo-ying,WANG Bao-hong,GUO Jin-jiang,LUO Yao(Department of Pediatrics,the Affiliated Hospital of Binzhou Medical University,Binzhou 256603,Shandong Province,China)  
Objective To explore the protective role of glutamine on lung injury induced by hyperoxia in neonatal rats.Methods Twenty-four 2-day-old newborn rats were randomly divided into air group(n=8),glutamine group(n=8) and hyperoxia group(n=8),among which the rats in air group were not processed.The rats in glutamine group were processed through intraperitoneal injection of 0.75 g·kg-1·d-1 glutamine solution before the experiment,and the rats in hyperoxia group were intraperitoneal injected with the same amount of 9 g·L-1 saline,and the above experiment was processed for following 3 days;the rats in hyperoxic group and glutamine group were located in the model case which had more than 900 mL·L-1 oxygen concentration,and in a same room,the rats in air group were located at atmospheric pressure in air.Through the statistical comparison,body mass of the each group at 3 days and 6 days were measured,separately.The rats were sacrificed after 6 days and lung tissue was removed to make paraffin specimen under aseptic conditions,then the expression of pathological changes and heat shock protein 70(HSP70) were observed.Results 1.In the 6th day after experiment,the survival rate in hype-roxia group was significantly lower than that in air group(P0.05),while between the glutamine group and the air group,there was no significant difference(P0.05).Exposing to hyperoxia for 3 days,the weight among 3 groups had no significant difference(P0.05),but when they were exposed to hyperoxia for 6 days,the weight of newborn rats in hyperoxia group was significantly lower than that in air group(P0.01);however,the glutamine group′ newborn rats had no significant difference compared with air group(P0.05).2.The pulmonary edema in hyperoxia group was the most serious,and the wet weight /dry weight values were significantly higher than those in air group(P0.05).However,the wet weight /dry weight values in glutamine group had no significant difference compared with air group(P0.05).3.Under the high oxygen exposure for 6 days,in hyperoxia group,there was intra-alveolar hemorrhage and a lot of exudative inflammatory cells,as well as a large number of protein powder dye liquid and severe congestive alveolar wall edema.In glutamine group,there was a very small amount of red blood cells in the alveolar oozing,and powder dye edema fluid presented on the alveolar cavity and alveolar wall was thickened.4.When they were exposed to hyperoxia for 6 days,HSP70 protein expression in hyperoxia group and glutamine group were ob-viously increased compared with that in air group(Pa0.01).HSP70 protein expression in the glutamine group was obviously increased compared with that in hyperoxia group(P0.01).Conclusions The survival rate of newborn rats under the situation of hyperoxia-induced lung injury can be significantly improved by the prophylactic use of the glutamine which is also beneficial to the development of newborn rat.In addition,HSP70 expression of newborn rats′ lung tissues under the situation of hyperoxia-induced lung injury can be increased by the prophylactic use of the glutamine.The protective role of glutamine in hyperoxia-induced lung injury is related to the expression of HSP70.
【Fund】: 山东省科技攻关项目(2009GG20002055);; 山东省优秀中青年科学家科研奖励基金(BS2010YY003);; 滨州医学院科研计划项目(BY2009KJ15)
【CateGory Index】: R722.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 6 Hits
1 HOU Wei, LIU Hai-Yan, LI Dan, ZHOU Jian-Ping, CHEN Xi. Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China;Expression of VEGF protein and mRNA in neonatal rats with hyperoxia-induced lung injury[J];Chinese Journal of Contemporary Pediatrics;2008-02
2 HUANG Xiao1,YANG Hui2,LUO Yao1,LIU Xiao-hong2,LIU Guo-sheng1,WU Xia1,WANG Zhi-jun1,XU Jing1(1.Department of Pediatrics,the First Affiliated Hosp ital,Jinan University,Guangzhou,510630,China; 2.Department of Neonatology,Children's Hospital of Shenzhen,Shenzhen,518026,China);Expression of STAT3 mRNA in the lung of neonatal rats exposed to hyperoxia and its significance[J];Journal of Jinan University(Natural Science & Medicine Edition);2010-04
3 XIA Shi-wen(Department of Neonatology,Maternal and Child Health in Hubei Province,Wuhan 430070,Hubei Province,China);Study Advances of Ventilator-Induced Lung Injury[J];Journal of Applied Clinical Pediatrics;2010-02
4 LIU Xiu-xiang1,ZHANG Hai-hong1,WAN Bin1,LUO Yao1,FENG Zhi-chun2(1.Department of Pediatrics,the Affiliated Hospital of Binzhou Medical University,Binzhou 256603,Shandong Province,China;2.Department of Neonatology,Bayi Children′s Hospital Affiliated to General Hospital of Beijing Military District,Beijing 100700,China);Growing Conditions of Type Ⅱ Alveolar Epithelial Cells and Their Transdifferent Identification in Vitro[J];Journal of Applied Clinical Pediatrics;2011-16
5 DING Yue,TANG Li-jun,HUANG Wei-min(Department of Neonatology,Nanfang Hospital of Southern Medical University,Guangzhou 510515,Guangdong Province,China);Progress of Diagnosis and Treatment of Bronchopulmonary Dysplasia in Premature Infants[J];Journal of Applied Clinical Pediatrics;2012-02
6 XUE Xin-dong,FU Jian-hua,CAI Qing;Research progress in the neonatal diseases[J];Chinese Journal of Practical Pediatrics;2010-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Jing,XU Feng,HU Lan,TAN Li-Ping,FU Yue-Qiang,FANG Fang,KUANG Feng-Wu,LU Zhong-Yi. Intensive Care Unit,Children's Hospital,Chongqing Medical University,Chongqing 400014,China;Expression and role of p38 mitogen-activated protein kinase in hyperoxia-induced lung injury juvenile rat model[J];Chinese Journal of Contemporary Pediatrics;2009-05
2 PAN Li,XU Wei,FU Jian-Hua,XUE Xin-Dong.Department of Pediatrics,Shengjing Hospital of China Medical University,Shenyang 110004,China;Effect of melatonin on hyperoxia-induced oxidant/antioxidant imbalance in the lung of neonatal rats with chronic lung disease[J];Chinese Journal of Contemporary Pediatrics;2009-07
3 LI Yan-fu(Department of paediatrics,The third people's hospital of Huizhou City,Ghuangdong Province,Huizhou 516002,China);High Risk Factor and Preventive Treatment Countermeasures of Premature with Bronchopulmonary Dysplasia[J];Chinese Journal of Medicinal Guide;2012-03
4 SUN Yan,WANG Jin-Rong,HAN Xiu-Zhen,LI Hua-Bing,SUN Li-Feng,CHEN Xing,FENG Yi-Zhen.Department of Pediatrics,Provincial Hospital Affiliated to Shandong University,Jinan 250021,China;Effects of budesonide on HIF-1α and VEGF expression and airway remodeling in an asthmatic mouse model[J];Chinese Journal of Contemporary Pediatrics;2012-08
5 MA Xing-Na;LI Qiu-Ping;FENG Zhi-Chun;Department of Neonatal Intensive Care Unit,Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command of People's Liberation Army;;Research progress in cytokines and signaling pathways for promoting pulmonary angiogenesis and vascular development[J];Chinese Journal of Contemporary Pediatrics;2013-09
6 LI Jing-jing1,ZHANG Yan-ting1,XU Li-hui2,MO Hong-bo1,OUYANG Dong-yun1,HE Xian-hui1 (1.Institute of Tissue Transplantation and Immunology,2.Institute of Bioengineering, College of Life Science and Technology,Jinan University,Guangzhou 510632,China);Down-regulation of STAT3 activation by AG490 enhances the inhibitory effects of cucurbitacin B on B16F10 melanoma cells[J];Journal of Jinan University(Natural Science & Medicine Edition);2011-06
7 ZHANG Pan,ZHANG Xiao-qun,QIU Jie,YANG Yang,ZHOU Xiao-yu*,ZHOU Xiao-guang(Department of Neonate,Nanjing Children's Hospital Affiliated to NJMU,Nanjing 210008,China);Preliminary study of the fetal rat alveolar type Ⅱ epithelial cell growth and ABCA3 expression changes[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2012-11
8 LIU Bo-yi, ZHENG Xiao-li, ZHENG Xiang, FANG Zhi-cheng, LIU Pei. Intensive Care Unit (ICU), Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei, CHINA;Influence of dexamethasone on the lung tissue cell apoptosis in rabbits with VILI[J];Hainan Medical Journal;2013-08
9 WU Chun-xue,CUI Ying,LI Yong,et al.Department of Emergency,Cangzhou Central Hospital,Cangzhou 061001,China;The Effect of Alanyl-Glutamine on HSP70 and MLIS and the Duration of Mechanical Ventilation in ALI/ARDS Patients[J];Chinese General Practice;2011-36
10 ZHANG Hai-hong,LIU Xiu-xiang,ZHAO Guo-ying,WANG Bao-hong,LUO Yao(Affiliated Hospital of Binzhou Medical University,Binzhou 256603,P.R.China);Protection of Glutamine on lung injury induced by hyperoxia in neonatal rats and its mechanism[J];Shandong Medical Journal;2012-21
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Bo LIU, Xue-Yan LIU, Juan LI, Wei WANG, Ke-Lun WEI. Neonatal Intensive Care Unit, Jinzhou Children′s Hospital, Jinzhou 121000, China;Exposure to prolonged hyperoxia decreases the expression of vascular endothelial growth factor in lungs of neonatal rats[J];The Chinese Journal of Contemporary Pediatrics;2005-05
2 HUANG Wan-Jie,XUE Xin-Dong,GUO Jing,YAO Li,FU Jian-Hua,QI Yong-Jun.Department of Pediatrics,Shengjing Hospital,China Medical University,Shenyang 110004,China;Early clinical presentations and MRI characteristics in newborns with cerebral infarction[J];Chinese Journal of Contemporary Pediatrics;2009-02
3 WANG Wei,QIAN Li-Ling,LIU Hai-Pei,SUN Bo Laboratory of Pediatric Respiratory and Critical Care Medicine,Children's Hospital andthe Institutes of Biomedical Sciences,Fudan University,Shanghai 201102,China;Engraftment of bone marrow stromal cells in lipopolysaccharide-injured mouse lungs[J];Chinese Journal of Contemporary Pediatrics;2009-05
4 WANG Qing-Hong, YANG Yu-Jia, CHEN Chong-Feng, YAO Yue, LI Meng. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China;Protective effects of delayed multiple course hyperbaric oxygen treatment against hypoxic-ischemic brain damage in neonatal rats[J];Chinese Journal of Contemporary Pediatrics;2009-06
5 SHI Jing,XIONG Ying,MU De-Zhi.Department of Pediatrics,West China Second University Hospital,Sichuan University,Chengdu 610041,China;Clinical evidence for the pharmacological therapy in newborn infants with hypoxic-ischemic encephalopathy[J];Chinese Journal of Contemporary Pediatrics;2009-09
6 WANG Xiao-Li,YANG Yu-Jia,XIE Min,YU Xiao-He,WANG Qing-Hong.Department of Pediatrics,Xiangya Hospital,Central South University,Changsha 410008,China;Hyperbaric oxygen promotes the migration and differentiation of endogenous neural stem cells in neonatal rats with hypoxic-ischemic brain damage[J];Chinese Journal of Contemporary Pediatrics;2009-09
7 DUAN Jiang1,2, CHEN Hong-Qing2, LI Ran2, YIN Xiao-Juan2, FENG Zhi-Chun21Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China, 2Bayi Children's Hospital, General Hospital, Beijing Military Area Command, Beijing 100700, China;Influence of TGF-β1 siRNA mediated by lentivirus vector on hyperoxia-induced lung injury in neonatal mice[J];Journal of the Fourth Military Medical University;2009-09
8 KONG Xiang-yong 1 , AN Jing 2 , FENG Zhi-chun 11 Department of Pediatrics, Zhujiang Hospital, First Military Medical Universi ty, Guangzhou 510282, China; 2 Depart-ment of Histology and Embryology, First Military Medical University, Guangzhou 510515, China;Electron microscopic observations of alveoli development and epithelial diff erentiation in the fetal lung of the Chinese[J];Journal of First Military Medical University;2004-01
9 MENG Ge1,ZHAO Jian1,L Xin-huai2,DING Ri-gao1(1.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China;2.Longkou People's Hospital,Longkou,Shandong 265701,China);Isolation,purification,primary culture and identification of alveolar type Ⅱ epithelial cell from rats[J];Bulletin of the Academy of Military Medical Sciences;2010-01
10 HUANG Yu-ge1,FENG Zhi-chun2,YU Yan-liang1,XIAO Fang-fang1(1.Department of Pediatrics,Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China;2.Bayi Children's Hospital,The Military General Hospital of Beijing PLA,Beijing 100700,China);Protective effects of MG-132 on p38 signaling pathway and cell apoptosis in lung injury induced by hyperoxia[J];Journal of Shanghai Jiaotong University(Medical Science);2009-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved