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《Journal of Applied Clinical Pediatrics》 2012-24
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Protective Effect of Glutamine on Lung Injury Induced by Hyperoxia in Neonatal Rats

ZHANG Hai-hong,LIU Xiu-xiang,ZHAO Guo-ying,WANG Bao-hong,GUO Jin-jiang,LUO Yao(Department of Pediatrics,the Affiliated Hospital of Binzhou Medical University,Binzhou 256603,Shandong Province,China)  
Objective To explore the protective role of glutamine on lung injury induced by hyperoxia in neonatal rats.Methods Twenty-four 2-day-old newborn rats were randomly divided into air group(n=8),glutamine group(n=8) and hyperoxia group(n=8),among which the rats in air group were not processed.The rats in glutamine group were processed through intraperitoneal injection of 0.75 g·kg-1·d-1 glutamine solution before the experiment,and the rats in hyperoxia group were intraperitoneal injected with the same amount of 9 g·L-1 saline,and the above experiment was processed for following 3 days;the rats in hyperoxic group and glutamine group were located in the model case which had more than 900 mL·L-1 oxygen concentration,and in a same room,the rats in air group were located at atmospheric pressure in air.Through the statistical comparison,body mass of the each group at 3 days and 6 days were measured,separately.The rats were sacrificed after 6 days and lung tissue was removed to make paraffin specimen under aseptic conditions,then the expression of pathological changes and heat shock protein 70(HSP70) were observed.Results 1.In the 6th day after experiment,the survival rate in hype-roxia group was significantly lower than that in air group(P0.05),while between the glutamine group and the air group,there was no significant difference(P0.05).Exposing to hyperoxia for 3 days,the weight among 3 groups had no significant difference(P0.05),but when they were exposed to hyperoxia for 6 days,the weight of newborn rats in hyperoxia group was significantly lower than that in air group(P0.01);however,the glutamine group′ newborn rats had no significant difference compared with air group(P0.05).2.The pulmonary edema in hyperoxia group was the most serious,and the wet weight /dry weight values were significantly higher than those in air group(P0.05).However,the wet weight /dry weight values in glutamine group had no significant difference compared with air group(P0.05).3.Under the high oxygen exposure for 6 days,in hyperoxia group,there was intra-alveolar hemorrhage and a lot of exudative inflammatory cells,as well as a large number of protein powder dye liquid and severe congestive alveolar wall edema.In glutamine group,there was a very small amount of red blood cells in the alveolar oozing,and powder dye edema fluid presented on the alveolar cavity and alveolar wall was thickened.4.When they were exposed to hyperoxia for 6 days,HSP70 protein expression in hyperoxia group and glutamine group were ob-viously increased compared with that in air group(Pa0.01).HSP70 protein expression in the glutamine group was obviously increased compared with that in hyperoxia group(P0.01).Conclusions The survival rate of newborn rats under the situation of hyperoxia-induced lung injury can be significantly improved by the prophylactic use of the glutamine which is also beneficial to the development of newborn rat.In addition,HSP70 expression of newborn rats′ lung tissues under the situation of hyperoxia-induced lung injury can be increased by the prophylactic use of the glutamine.The protective role of glutamine in hyperoxia-induced lung injury is related to the expression of HSP70.
【Fund】: 山东省科技攻关项目(2009GG20002055);; 山东省优秀中青年科学家科研奖励基金(BS2010YY003);; 滨州医学院科研计划项目(BY2009KJ15)
【CateGory Index】: R722.1
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