Study of cell-medjated immunity of infantile human cytomegalovirus hepatitis and effets of interferon for the disease
TANG Se;XIONG Ying;CHENG Yong-Xiu;YAO Yu-jia(Department of pediatrics, Second Affiliated Hospital,West China University of Medical Science)
Objectives To research function of cell-mediated immunily and effects of interferon in treating infantile human cytomegalovirus (HCMV) hepatitis.Methods Three methods, included a specific probe to hybridize with DNA of human cytomegalovirus (HCMV), separating the HCMV from urine and culturing them, serum ELISA-CMV-IgM, were used in diagnosis of 87 infants with hepatitis syndrome. The 69 cases with HCMV hepatitis were confirmed.Interleukin (IL-2), CD3, CD4, CD8 and CD4/CD8 ratio of peripheral blood leukocytes of 22 cases among the 69 cases were investigated in different clinical stages. The 35 cases were treated with interferon together with daily vitamin C and inosine therapy intravenously, other 34 cases were only treated with daily vitamin C and inosine therapy for control.Results The cell-mediated immunity was imparted in in infants with HCMV hepatitis. Athough the patient have clinical improvements accompanied with clevation values of IL-2 and CD3, CD4 and CD8were sitll abnormal during convalescence. There as a significant difference in clinical symptoms disappearance between the two groups. The average alleviating time of jaundice, hepatomegaly and serum SGPT level after treatment with γIFN were 14.9, 28.2 and 14.9 days ahead of those in the control group. The cure rate at one month and two months after treatment was 48.6% and 88.6% in th γIFNgroup; 20.6 % and 47.1% in the control group.Conclusions The function of cell-mediated immunity was wrecked in infantile human cytomegolovirues hepatitis. The γ-IFN was effective in treatment of HCMV hepatitis.