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《Journal of Shaoyang University(Natural Science Edition)》 2018-01
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TSG Alleviates TNF-α Induced Human Brain Microvascular Endothelial Cells Damage by Inhibiting NF-κB Signaling Pathway

HE Shaobo;JIANG Chuanming;YANG Qin;ZHANG Caiping;LONG Shiyin;Department of Biochemistry and Molecular Biology,School of Pharmacy and Life Sciences,University of South China;School of Medical Laboratory Technology,Shaoyang University;  
Objective This study aims to investigate how 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside(TSG) protects TNF-α induced human brain microvascular endothelial cells(HBMECs) damage mediated by N F-κB signaling pathway.Methods To test the effect of TSG or NF-κB inhibitor pyrrolidine dithiocarbamate(PDTC) on cell activity and NF-κB p65/caspase-3 expression,HBMECs were cultured in vitro and pretreated with different concentrations of TSG or PDTC before stimulation with TNF-α.Results Western-blot results showed that the expression of NF-κB p65 and caspase-3 increased significantly upon TNF-α stimulation,while the expression of which obviously decreased when treated with TSG.Compared to the group only stimulated by TNF-α,the cell activity of HBMECs clearly increased when treated with both TNF-α and TSG or PDTC.Interestingly,the cell activity of HBMECs was in a PDTC dose-dependent manner,which had significant difference in 80 and 160μmol/L group compared with that of TNF-α group(P 0.05).Conclusion TSG is able to protect TNF-α-induced human brain microvascular endothelial cells damage by inhibiting NF-κB signaling.
【CateGory Index】: R743.3
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