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Isolation and identification of chemical constituents from pine pollen

TANG Yu,ZHANG Yu,YUAN Jiu-zhi,MA Yue-ping(School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)  
Objective To study the chemical constituents of pine pollen(the pollen of Pinus massoniana Lamb.).Methods Compounds were isolated and purified repeatedly by silica gel,Sephadex LH-20 and ODS column chromatography,and their chemical structures were elucidated on the basis of the physicochemical properties and spectral analysis.Results Nine compounds were obtained and identified as naringenin(1),taxifolin(2),dihydrokaempferol(3),kaempferol(4),chalconaringenin(5),isorhamnetin-3-O-β-D-glucopyranoside(6),kaempferol-3-O-β-D-glucopyranoside(7),tyrosol(8),and rhododendrol(9).Conclusions Compounds 1,2,5-9 are isolated from pine pollen for the first time.
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