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《Practical Preventive Medicine》 2007-02
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Better Strategy for Proteomic Analysis at Different Stages in Chronic Myeloid Leukemia

YAO Feng,LI Rong,LIU Xiao-li,et al.(Department of Hematology,Nanfang Hospital of Nafang Medical University,Guangzhou 510515,Guangdong,China)  
Objective To compare differentially expressed proteins in bone marrow cells at chronic and blast phases of chronic myeloid leukemia by means of different grouping methods,and to screen the proteins involving in the transformation.Methods Two-dimensional electrophoresis(2-DE) was used to separate proteins and compare the changes of proteins expression in bone marrow cells at these two stages with three grouping methods,including the mixing samples group,the random samples group and the individual group.The differentially expressed protein spots were identified by peptide mass fingerprint in combination with database searching.Results The protein spots of the two stages were resolved with a match rate of 70.0678% in the mixing samples group;6 gel pages from CP and BC respectively were matched by pairs in the random samples group,and the range of match rate was from 43.2604% to 58.3477%.The match rate was 74.0988% in the individual group.The common differentially expressed protein spots from the mixing samples group and the random samples group were 103,and χ2 test result was P0.05.Twenty protein spots were selected from the co-expressed different spots among these 3 groups for identification by peptide mass fingerprint in combination with database searching,and 15 spots were identified.Conclusion Distinct protein profiles can be obtained by using the mixing bone marrow cells in the comparative proteomics analysis.The distinction of individuals can be eliminated efficiently by this method and the steps of experiment can be simplified.
【Fund】: 国家自然科学基金资助项目(编号:30271463);; 广州市科技计划项目(编号:2006E3-E0401)资助课题
【CateGory Index】: R733.7
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【References】
Chinese Journal Full-text Database 1 Hits
1 ZHU Hong-qian1,LIU Xiao-li,LI Rong,DU Qing-feng,ZHANG Hao,GONG Jun-mei,OUYANG Zhao(1 Nanfang Hospital,Nan Fang Medical University,Guangzhou 510515,P.R.China.);A study on the protein expression change of heterogeneous nuclear ribonucleoprotein K in CML-CP and CML-BC[J];Shandong Medical Journal;2007-34
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Rong,LIU Xiao-Li,DU Qing-Feng,ZHANG Song,ZHOU Shu-Yun Department of Hematology,Nanfang Hospital,The First Military Medical University,Guangzhou,Guangdong,510515,P.R china;Two-dimensional Polyacrylamide Gel Electrophoresis Analysis of Apoptosis in K562 Cells Induced by Harringtonine[J];Chinese Journal of Cancer;2004-10
2 LI Rong, LIU Xiao-li, DU Qing-feng,ZHANG Song, LUO Rong-cheng, ZHOU Shu-yun. Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Proteome analysis of apoptotic K562 cells induced by harringtonine[J];Chinese Journal of Hematology;2004-06
3 WANG Li Shun, YIN Yan Hui, GAO Su Jun et al. Department of Immunology, School of Basic Medical Sciences, Jilin University, Changchun 130021,China;New strategy of two-dimensional gel electrophoresis-based proteomics analysis in esophageal cancer[J];Chinese Journal of Immunology;2003-04
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Shuang1, LU Li-chun2, FANG Wei-yi1, YAO Kai-tai1 1Oncology Institute, Key Lab of Molecular Tumor Pathology of Guangdong Province, Southern Medical University, Guangzhou 510515, China; 2Department of Pathology, 458 Hospital of PLA, Guangzhou 510602, China;Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues[J];Journal of Southern Medical University;2007-02
2 Meng Gen,Wang Hailong,Liu Bingyu,Wang Hongli,Li Weihua,Wang Hongxia,Wei Kaihua~*,Zhang Xuemin,Yang Songcheng(National Center of Biomedical Analysis,Beijing 100850)Cen Jian(Department of Hematology,General Hospital of Navy,Beijing 100037);Identification of Protein Mixtures from K562 Cell by Nano Spray Capillary Liquid Chromatography Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2005-11
3 LIU Cha-zhen, ZHU Pei-yun, SHI Min-xin, LIU Ji-bin, WANG Wen-jing (Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China; Nantong Tumor Hospital, Jiangsu Nantong 226361, China);Technique of Expression Proteomics and Progress of Studies on Esophageal Cancer Proteomics[J];Journal of Environmental & Occupational Medicine;2006-02
4 HUANG Zhi-yong1, ZHAO Liang2, ZHANG Jun1, XIONG Gang1, WANG Wu-jun11.Cardiothoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P.R.China2.Department of Pathology, Southern Medical University, Guangzhou 510515, P.R.China;Two-dimensional gel electrophoresis-matrix assisted laser desorption/ionization time-of-flight mass spectrometry to screen tumor-related proteins in esophageal carcinoma[J];Chinese Journal of Cancer Prevention and Treatment;2007-12
5 LI Jun-cai1 FU Zhong-xue1 WANG Bin1 DONG Pu-jiang1 HUANG Kai-shun2(1 The First Affiliated Hospital, Chongqing Medical University,Chongqing 400016,China)(2 Institute of Basic Medical Sciences, Chongqing Medical University,Chongqing 400016,China);Differential Expression of 14-3-3 protein ε,S100A9,in Cancerization of Human Esophageal Epithelial Cells[J];China Biotechnology;2008-02
6 Xiang-Lan Li1, Jin Chang1, Hong-Tao Hu1, Qing-Shan You*, Yu-Yan Ma2 (1Department of Radiation Oncology, 2Tumor Institute, Tumor Hospital, Harbin Medical University, Harbin 150081, China);Detection and Analysis of Proteomic Patterns of Human Adenocarcinoma Cell Lung Cancer A549 Treated with Radiation[J];Chinese Journal of Cell Biology;2008-05
7 LIU Cha-zhen, ZHU Pei-yun, SHI Min-xin, LIU Ji-bin, WANG Wen-jing, LIAO Ping, XIANG Cui-qin, ZHANG Yi-xin (Public Mollecular Biology Researching Office,Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336,China);Screening of serum proteome biomarker of esophageal squamous cell carcinoma by WCX2 protein chip[J];China Oncology;2007-09
8 LI Zu-guo ZHAO Liang LIU Li DING Yan-qing Guangdong Province-Ministry of Education Key Co-Laboratory of Functional Proteomics and Transcriptomics Related to Major Diseases,Key Laboratory of Molecular Tumor Pathology of Guangdong Province,Department of Pathology of Southern Medical University,Guangzhou 510515,China;Monitoring changes of serum protein markers in metastatic colorectal carcinoma model[J];Chinese Journal of Pathology;2007-01
9 Zhong Xiaolan~1,Lv Zhiping~1,Qian Lingjia~2,et al.(College of TCM,Southern Medical University,Guangzhou 510515;2.Institute of Hygiene Environmental Medicine,Tianjin 300050);Study on Differential Expression of Proteome in the Liver Tissue of the Model Rat with Stagnation of the Liver-qi Induced by Restraint[J];Journal of Traditional Chinese Medicine;2006-05
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Rong, LIU Xiao-li, DU Qing-feng, TIAN Hong, SONG Lan-lin, ZHANG Yan, YANG Yi, ZHOU Shu-yun Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510015, China;Experimental study of K562 cell apoptosis induced by harringtonine[J];Journal of First Military Medical University;2002-09
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