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《Practical Preventive Medicine》 2007-02
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Primary Culture and Identification of Rabbit Myometrial Smooth Muscle Cells

ZENG Run-qing,WANG Ruo-guang,LIU Hui-ping,et al.(Department of Integrated Traditional Chinese and Western Medicine,Hunan College of Traditional Chinese Medicine,Changsha 410007,Hunan)  
Objective To study the two methods of primary culture of rabbit myometrial smooth muscle cells(MSMC) and to identify their partial biological properties.Methods Rabbit myometrial tissue was planted and MSMC was digested by Collagenase I.The cellular morphology was observed by inverted phase contrast microscope and the nucleus and cytoplasm were observed after haematoxylin and eosin (HE) staining.The immunocytochemistry was used to identify the MSMC.ResultsThe cells were successfully cultrured by these two methods and grown in the "peak—valley" mode.The cultrured MSMC were fusiform shape with a big oviform or round nuclei,and rich in cytoplasm.Immunohistochemical study revealed strong expression of α-smooth muscle actin(a—SMActin). The purity of the MSMC was above 99% after passage and purification,and no abnormality was seen.Conclusion We have successfully harvested the primary cultured rabbit myometrial smooth muscle cells and obtained monomorphic cells with stable growth.In addition, the method is convenient,economical and practical,and the culture and sublimation could be in synchronism.
【Fund】: 国家自然科学基金项目(30572405)
【CateGory Index】: R-332
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