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《Practical Preventive Medicine》 2007-02
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Rreleasing of Cyt-c From Neuronal Mitochondria and Activation of Caspase3 in Cytoplasm After Intracerebral Hemorrhage in Rat Models

XIONG Xin-gui,LIANG Qing-hua,BAO Tai-cheng,et al.(Institute of Combined Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University,Changsha 410008,Hunan,China)  
Objective To investigate the impaired mechanism in neurons around the haematoma after cerebral hemorrhage through observing the releasing of Cyt-c(Cytochrome c) from mitochondria of neurons around the haematoma and the information of activated Caspase-3(cysteinyl aspartate specific proteinase-3) in cytoplasm.Methods Ⅻ type collagenase was stereotaxically injected into the globus pallidus of rats to establish the ICH model.Immunohistochemical method was used to exam the expression of Cyt-C and activated Caspase-3 after ICH in rat at different time points.Results Cyt-c was began to release in the neuron around the hematoma after ICH in rat at 6 hours after operation,there were some positive cells stained in the cytoplasm around the hematoma;1 day thereafter,the cytoplasm was stained strongly,the cell shape became abnormal,and the number of positive cells reached the peak;and at the 3rd day,the staining began to fade,the positive cells decreased;at the 5th day,the staining continually reduce and the positive cells declined.The activated Caspase-3 in cytoplasm appeared around the hematoma at 6 hours after operation,and peaked at the 1st day,then descended lightly at the 3rd day,furthered at the 5th day.As compared with the control group,the difference was significant(P0.01).Conclusion The releasing of Cytc from neuronal mitochondria and the activation of Caspase-3 in cytoplasm appeared quickly around the hematoma after ICH and these results suggest that they play impotrant roles in the neuronal apoptosis after ICH.
【Fund】: 湖南省自然科学基金(01JJY2024);; 省科技基金(1013-50)
【CateGory Index】: R743.34
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