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《Practical Preventive Medicine》 2007-02
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Study on Experimental Rabbit Model of Hepatic Fibrosis Induced by High-sucrose/High-fat Diet

LUO Jie,XU Hui,LIU Yi(Department of Biochemistry, Changsha Medical College,Changsha 410219,Hunan,China)  
Objective To induce hepatic fibrosis in New Zealand white rabbits by feeding with high-sucrose/high-fat diet and to study the effects of diet on hepatic fibrosis.Methods Male New Zealand white rabbits were randomly divided into two groups: the control group(n=10,fed with normal fodder) and the model group(n=10,fed with 37% sugar,10% lard and 53% normal fodder). After an overnight fasting,the blood samples of the rabbits were tested for the levels of plasma glucose and triglyceride once a month.At the end of the 5th month,all of the rabbits were sacrificed.The levels of hydroxyproline(Hyp) were measured in liver tissue.The degrees of hepatic steatosis,inflammation,and fibrosis were assessed with HE and VG stains.The protein expression of type Ⅳ collagen and transforming growth factor β1(TGF-β1) were determined by immuno-histochemical method.Results The plasma triglyceride levels from the 1st month and glucose levels from the 3rd month increased significantly with the feeding duration in the model group in comparison with the control group(P0.05).The level of Hyp increased,and hepatocyte steatosis,lymphocyte infiltration and proliferated collagen fibers were observed in the liver tissues of the model group. The protein expression of type Ⅳ collagen and TGFβ1 was increased in liver tissue of the model group.There were no significant changes in liver histology of the control group,however.Conclusion High-sugar and high-fat diet induces hepatic toxicity,and increases the protein expression of TGF-β1 and type Ⅳ collagen,which can induce early-stage hepatic fibrosis in New Zealand white rabbits.
【Fund】: 湖南省教育厅科研项目(05C069)
【CateGory Index】: R-332
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