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《Practical Preventive Medicine》 2007-02
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Effect of Alcohol on Serum NOS Activity and ADMA Concentration in Rat Models

LI You,HE Bin-Qi,LI Ying,et al.(Department of Public Health,Nanhua University,Hengyang 421001,Hunan,China)  
Objective To study the effect of alcohol on serum ADMA level and NOS activities in rat models.Methods Sprague-Dawley male rats weighing about 250g were actively given with three doses of alcohol(14V/V,28V/V,and 56V/V) by intragastric feeding at a rate of 1mL/100g,one time per day for two months.On the 60th day,blood samples were collected from femoral arteries,centrifuged(3500rpm,10min) and separated immediately.Serum levels of MDA(thiobarbituric acid method),NO(Griess method) and ADMA(HPLC) were determined.Serum activity of NOS(biochemical approach) was determined.The experimental rats were randomly divided into 7 groups:(1) negative control group,rats were given with equal volume of distilled water;(2) the alcohol groups,rats were given with different concentrations of alcohol(Hongxing Erguotou,14V/V,28V/V,and 56V/V);(3) high fat diet group,rats were given with high fat diet;(4) high fat diet alcohol at intermediate concentration group(28V/V);(5) VitE(100mg/kg) protected group,rats were given with Vitamin E at 1 hour before intragastric alcohol administration,while high fat diet was fed.Results Alcohol and/or high fat diet increased the serum levels of MDA,NO and ADMA,inhibited the activity of eNOS and increased the activity of iNOS.Vitamin E ameliorated the injurious effects induced by alcohol and high fat diet.Conclusion Alcohol is an important risk factor of hyperlipoidemia and endothelial damage.It induces the production of the blood lipid and lipid peroxidation,interferes with the metabolism of NO.The injurious mechanism induced by alcohol is related to the lipid peroxidation and the serum level of ADMA.Vitamin E can protect against the injurious effect induced by alcohol and high fat diet.
【Fund】: 湖南省卫生厅科研课题资助(B200508)
【CateGory Index】: R363
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