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《Practical Preventive Medicine》 2007-02
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Comparative Study of in Vitro and in Vivo Anti-Oxygen Free Radical Effects of Different Parts of Portulaca oleracea L

YU Qing-gao,WANG Xiao-chun,XIONG Zhi-qing (Xiangtan Vocational & Technical College,Xiangtan 411100,Hunan)  
Objective The experiment is designed to study the in vitro and in vivo anti-oxygen free radical effects of the different parts of Portulaca oleracea L including its leaves,stems and roots.Methods In the in vitro experiment,the measurement of·OH was made with the oxidation of phenathroline-Fe2+,and that of O-·2 with the improved pyrogallol auto-oxidation method.Rabbits were used in the in vivo experiment,raised with the leaves,the stems and the roots of Portulaca oleracea L,and 10 weeks later their serum SOD MDA were evaluated.Results The in vitro experiment showed that all of the leaves,stems or roots of Portulaca oleracea L had the effect of anti-oxygen free radical,and the effects of the stems and the roots were more powerful than that of the leaves,there were significant differences.The in vivo experiment with rabbits showed that the stems and the roots had the effect of anti-oxygen free radical,but not the leaves.Conclusion The stems and the roots of Portulaca oleracea L have a strong effect of anti-oxygen free radical in vitro and in vivo,while its leaves has the effect only in vitro.
【CateGory Index】: R285
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