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《Reproduction and Contraception》 2003-06
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Effect of Human Endometrium Cells on Early Mouse Embryonic Development in Vitro

Yan ZHANG, Hong SHI (Department of Obstetrics and Gynecology, First Affiliated Hospital,Dalian Medical University, Dalian, 116011)  
Objective;To study the early mouse embryos development in a human endometrium cells co-culture system and that in a medium alone in vitro. Methods:Two-cell mouse embryos were cultured with or without the human endometrial cell line in DMEM containing 0. 3% BSA, respectively. The percentage of embryos developed to 4- to 8- cell stage, morula, blastocyst transformation, expansion, hatching, and implantation were compared. Results: Percentages of the co-cultured embryos attained to the 8-cell stage (75.0% vs. 31. 4%), morula (68.3% vs. 24. 8%), blastocyst (50.8% vs. 11.4%), and hatching (36.7% vs. 0%) were significantly higher than that in the DMEM, P0. 01. The implantation rate of co-cultured embryos also significantly increased as compared to controls (25. 0% vs. 3. 1%, P0. 05). Conclusion: The human endometrium cells co-culture system had postive effect on mouse embryo development by improving quality of blastocyst and increasing implantation rate.
【CateGory Index】: R711.6
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【Co-references】
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