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《Reproduction and Contraception》 2003-06
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Immunochemical Localization and Enzymatic Activity of Both Inducible and Endothelial Nitric Oxide Synthase in Mouse Oviduct

Jia-xin WANG, Zhan-zhong JIN, Zeng-yi WANG, Xiang-hao KONG, Xiao-hong CUI ( Laboratory of Reproductive Immunology, Hebei Beifang College, Xuanhua, 075131) ( The Hospital of Xuanhua Iron and Steel Company, Xuanhua, 075100)  
Objective:To investigate the expression patterns and enzymatic activity of both inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in cycling oviduct. Methods :Indirect im-munohistochemical protocol was employed for this study with assistance of computer imaging analysis system. Results:An extremely similar expression pattern of both iNOS and eNOS was detected in the oviducts during the estrous cycle. In dioestrus , a weaker positive reaction showed at the epithelial cells of most villi while a few of positively stained cells localized at the basal segments of villi. The number of both iNOS and eNOS positive cells and their intensity in mucosa increased gradually during the cycle. However, the highest intensity of iNOS and/or eNOS in immunohistochemical reaction appeared in metoestrus. The detection of 3-NT in cycling oviduct showed the same pattern with that of NOSs. Conclusion: This paper provides the evidence that both the expression patterns and enzymatic activity of iNOS and eNOS in oviduct are significantly characteristic of estrous cycle in mice. The highest intensity of immunohistochemical reaction and the highest activity of NOSs in metoestrous oviduct may indicate that oviduct functions in such a way would be one of the natural reasons why fertilization always takes place at oviduct in mammals.
【Fund】: 河北省自然科学基金资助项目(398438)
【CateGory Index】: Q819
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