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《Reproduction and Contraception》 2003-06
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Oocyte Retrieval and Maturation from Cryopre-served Human Ovarian Cortex in Nude Mouse

YL GUO,Ke-jun GUO,Xiao-guang TONG, Li NIE,Ming-yan GAO ( Department of Gynecology, First Affiliated Hospital of China Medical University, Shenyang, 110001) ( Department of Obstetric and Gynecology, Tie Xi Hospital of Anshan City, Anshan, 114012) (3. Department of Obstetric and Gynecology, Shenyang 242 Hospital, Shenyang,110034)  
Objective: To investigate the potential of transplantation of frozen-thawed human ovarian tissue for oocyte retrieval and mturation in nude mouse. Methods: Ovarian tissues tissues from 5 patients were removed, dissected, and stored in 1. 5 mol/L DMSO+10% serum with a programmable freezer and transferred to liquid nitrogen. Three months later, 89 nude mice were transplanted with thawed ovarian cortex fragments, stimulated with hFSH injections for 12 weeks. Oocyte retrieval were performed 36 h after hCG injection with 18# needles. Results: A total of 19 oocytes were obtained from 3 to 9 mm follicles in nude mice and were matured in vitro in TC-199 medium with 20% FBS serum, 25 mmol/L pyruvic acid, and 75 mIU/mL FSH and LH. Totally 15 secondary oocytes were retrieved in the metaphase stage. Conclusion: Human ovarian follicles could survive cryopreservation and used as long-term xenograft. It is feasible to have oocyte retrieved and maturized from cryopreserved human ovarian cortex transplanted into mouse.
【CateGory Index】: R321
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