The Determination of Carbohydrate by HPLC
Liu Yongqing and Han Shanying Taiyuan Forage Institute
The carbohydrate was determined by HPLC. The optimum of analysing high maltose is that 79% acetonitrile is used as moving phase, flow rate is 1. 0 ml/min and column temperature is 40℃. The con-tent of maltose in new梩ype sweetener is up to 70. 20%. The quantitative analysis is of good repeatability, variation coefficient is less than 1% and recovery is 98. 25% ~106. 00% when 83% acetonitrile is used as moving phase to separate carbohydrate from huney. This study provides the basis for standard analysis of car-bohydrate.
【CateGory Index】： O657.7