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《Journal of Tianjin Normal University(Natural Science Edition)》 2007-01
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Analysis of Genetic Diversity in Populations of Two Commercial Species of Mercenaria mercenaria and Cyclina sinensis(Bivalvia Veneridae) Based on ITS-1 Sequence

WU Qi,PAN He-ting,PAN Bao-ping(College of Chemistry and Life Science,Tianjin Normal University, Tianjin 300074,China)  
The genetic diversity of cultured Mercenaria mercenaria and wild Cyclina sinensis populations was investigated by using the ribosome DNA gene interval transcribed spacers1(ITS-1)sequences.After PCR amplification,ligation and sequencing,the ITS-1 sequences of M.mercenaria and C.sinensis obtained from individuals of populations ranged in 718~724 and 665~683 nucleotides in length,respectively.By alignment with Clustal-X,725 homologous sites in ITS-1 fragment of M.mercenaria had 714 monomorphic sits.Its nucleotide diversity Pi=0.003 02,Theta(per site) Eta=0.003 04, and 4 haplotypes were identified from the population,analyzing the sequences variations with DNAsp4.695 homologous sites in fragment of C.sinensis had 644 monomorphic sits.Its Pi= 0.030 71,Eta=0.037 8 and 6 haplotypes were identified from population.The pairwise genetic distance matrix of haplotypes was produced by PAUP 4.10 beta following the method of Hasegawa,et al(1985) to evaluate the ratios of transition/transversion in nucleotides.The results showed that the genetic distance among haplotypes of M.mercenaria and C.sinensis were 0.001 4 to 0.005 6 and 0.003 6 to 0.010 5 respectively,the later of index is much higher than the former.The influencing factors of genetic diversity in the populations and the conservation of idioplasm were also discussed.
【Fund】: 天津市科委应用基础研究项目(06YFJMJC11800);; 天津师范大学第5届“创新杯”学生科技重点项目
【CateGory Index】: S917.4
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