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In Situ Hybridization of Hepatitis C Virus RNA in Liver Tissue of Paticnts with HCV Infection

Zhao Xiping; Tang Zhenya; Yang Dongliang et al (Clnical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wuhan 430030)  
To study the location of hepatitis C virus (HCV) in liver and its relationship with the inflammation of liver tissue, we detected HCV RNA in liver tissue of a group of patients with HCV infection by in situ hybridization (ISH). Results: Among the 12 patients, 9 (75%) were positive for HCV RNA; The staining signal was located mainly in the cytoplasm of liver cells ; The specificity of the result was demonstrated by pretreating the positive sectipns with RNase and DNase, respectively. As expected, the positive signal was disappeared after RNase treatment, but not affected by DNase treatment. The positive cells can be distributed in or near the necroinflammatory area, but most f them had no apparent topographical relationship with necroinflammation. The results suggest that ISH is a useful method in the study of physiological characteristics and pathogenesis of HCV.
【Fund】: 国家八·五攻关资助!No.85-916-01-05-6
【CateGory Index】: R512.6
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Chinese Journal Full-text Database 1 Hits
1 Yan Fuming, Hao Fei, Gu Changhai (Department of Infectious Disease, Southwest Hospital, Third Military Medical Univesity, Chongqing,400038);Study of expression of hepatitis C virus antigens in the liver from patients with severe viral hepatitis[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;1998-05
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 YAN Fuming ,HAOFei,CHENAnshen ,etal. DepartmentofInfectiousDiseases ,the 16 1stCentralHos pitalofChinesePLA ,Wuhan 430 0 10 ,China;Extrahepatic infection and replication of hepatitis C virus in patients with severe hepatitis C[J];Chinese Journal of Infectious Diseases;2001-05
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