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Influence of Drynaria on Physicochemical and in vitro Biological Properties of Calcium Phosphate Cement

JIANG Xiao-Xin~1,QU Shu-Xin~2,LIN Sun-Zhong~2,DUAN Ke~2,WENG Jie~2 (1.School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;2.Key Lab of Advanced Technologies of Materials,Ministry of Education,School of Material Science and Engineering,Southwest Jiaotong University, Chengdu 610031,China)  
The extract of drynaria,a traditional Chinese medical herb,was added into dicalcium phosphate dihydrate (DCPD) with concentration of 5wt%,10wt%and 15wt%to form drug-loaded calcium phosphate cement (CPC).The effects of drynaria on the physichemical properties and drug release behavior of CPC were characterized by Gilmore needle tests,X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),compressive tests,scanning electron microscope(SEM) and UV-Vis spectrophotometry(UV-Vis).The effects of drynarialoaded CPC on the morphology,proliferation,and differentiation of MC-3T3 osteoblast were evaluated by SEM, Alamar blue assay,and alkaline phosphatase assay,respectively.It was found that the addition of drynaria increased the setting time and compressive strength of CPC in dose-dependent manners.The addition of drynaria also accelerated the initial hydration of CPC,but inhibited the subsequent conversion ofα-tricalcium phosphate,which were closely related to the concentration of drynaria.In comparison,the phases of CPC hydration products were not affected by drynaria.Drynaria-loaded CPC consisting of platy and acicular crystals were more compact than the control CPC.The drug release profile exhibited an initial fast release stage followed by a slow release stage,which was fit to the diffusion-controlled Higuchi model.The effect of drynaria-loaded CPC on osteoblasts was time- and dose-dependent.CPC loaded with 5wt%and 10wt%drynaria significantly promoted osteoblast proliferation after culture for 5d(P0.01).After cultured with drynaria-loaded CPC for 7d,osteoblasts proliferated steadily.Osteoblasts cultured on drynaria-loaded CPCs and control CPC showed similar differentiation.Osteoblasts with a normal morphology were observed on the surface of drynaria-loaded CPC,indicating an excellent biocompatibility.
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