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《Journal of Wuhan University(Natural Science Edition)》 1987-01
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STUDIES ON PROTOPLAST FORMATION, REGENERATION AND FUSION OF CORYNEFORM BACTERIA

Kao Zhongxiang and Chen Shuyun  
Protoplasts were prepared with giycine-penicillin pretreatment and lysozyme treatment. The formation frequency of protoplasts was highest when penicillin was added in midexponential growth phase. The regeneration of protoplasts was affected by EDTA, penicillin preteatment and the physiological state of the bacteria, Both the soft agar method and the addition of Bovin Serum Album in the lysozyme solution and 1he dilution fluid can enhance the frequency Of regeneration. The intraspecific fusants were obtained with indirect selective method, The fusion freqnency was 6.8×10~(-7) of plated protoplasts.
【Fund】: 中国科学院科学基金
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Jiang Xingjuan Yang Qingyun" Een Darning Feng Dexin Ye Yinfeng Sheng Zujia(Institute of Genetics, Fu Dan University, Shanghai);The Transfer of Fiasmid through Cell Fusion between Bacillus subtilis[J];Acta Genetica Sinica;1981-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Ming, SHUANG Bao, LI Haitao, WANG Qing, GAO Jiguo(College of Life Sciences, Northeast Agricultural University, Harbin 150030, China);Progress and application of Bacillus subtilis in different fields[J];Journal of Northeast Agricultural University;2009-09
2 Yang Qingyun, Jiang Xingjuan, Yang Guochen;THE REGENERATION CONDITION IN A THERMOPHILIC BACTERIA[J];Journal of Fudan University;1985-04
3 Ping Wenxiang Zhou Dongpo Cao Zhongjun Hong Jianzhong Li zhanying ( Biology Department );METHODOLOGY RESEARCH FOR PROTOPLAST FUSION OF Bacillus subtili[J];;1989-03
4 ZHANG Dan-lin;WANG Wen-hao;ZHANG Xian-hao;CHEN Rui-ai;SU Dan-ping;HE Dong-sheng;South China Agriculture University;Lab of Veterinary Biologics R&D of Guangdong Province Enterprise,Guangdong Dahuanong Animal Health Product Co.,Ltd;;Establishment of Fusion Strain R2 of Swine Escherichia coli Expressing Omp 1 and Omp 2[J];Progress in Veterinary Medicine;2013-10
5 Liao Jin-song 1 Guo Yong 1 Zhuang Gui 2 (1. College of Food and Biological Engineering, South China Univ. of Tech., Guangzhou 510640, Guangdong, China; 2. Dept.of Biological Engineering,Zhengzhou Institute of Tech., Zhengzhou 450052, Henan, China);Preparation and Fusion of the Strain Protoplast for the Degradation of Polyvinyl Alcohol[J];Journal of South China University of Technology(Natural Science);2004-05
6 WU Kong xing 1, HUANG Qing yun 2,QIU Jia jun 3 (1 Guangdong Wen's Foodstuff Group Ltd., Xinxing 527439, China; 2 College of Veterinary Medicine, South China Agric. Univ.,Guangzhou 510642, China; 3 Guangdong Zhengda Kangdi Ltd., Guangzhou 51000;Development of 2-Combined Attenuated Strain F_4 by Fusing Escherichia coli. O_( 78) with Pasteurella multocida C_(48-1)[J];Journal of South China Agricultural University;2002-01
7 Li Yueqin;Zhou Tianhong;Liu Feipeng;Wen Jin(Dept.of Biology,Jinan University,510632,Guangzhou);ELECTROTRANSFER OF BACTERIAL PLASMID DNA BETWEEN DIFFERENT BACTERIAL SPECIES[J];JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION);1994-01
8 LIU Yi-chen, NI Xue-qin , WANG Shu-fang, HE Ming-qing, LI Hong-wei (l .Livestock Science & Technology College,Henan University of Science and Technology,Luoyang,471003,China; 2.College of Animal Science & Technology ,Sichuan Agricultural University, Ya'an 625014,China);Resistance gene for plasmid pBL29 of B.licheniformis 29[J];Journal of Henan University of Science and Technology(Agricultrual Science);2003-01
9 LIAO Jin-song~1,GUO Yong~1,ZHUANG Gui~2 (1.Food and Biological Engineering College,South China University of Technology,Guangzhou 510640,China; 2.Biological Engineering Department,Zhengzhou Institute of Technology,Zhengzhou 450052,China);Study on protoplast culture and fusion of strain in degrade polyvinyl-alcohol[J];Wool Textile Journal;2004-05
10 Dai Meixue 1) Zu Aimin 2) ( 1)Department of Biology,Shandong Normal University,250014,Jinan,PRC; 2)Department of Biology,Jinan Education Academy,250001,Jinan,PRC );STUDIES ON PROTOPLAST FORMATION AND REGENERATIONOF BACILLUS THURINGIENSIS STRAIN SD-5[J];JOURNAL OF SHANDONG NORMAL UNIVERSITY (NATURAL SCIENCE);1996-03
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