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《Journal of Wuhan University(Natural Science Edition)》 1990-01
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STUDIES ON ALKALINE PECTINASE FROM ALKALOPHILIC BACTERIA Ⅰ.Selection of Strains and Studies on the Culture Conditions for Enzyme Producing and Enzyme Properties

Huang Shijian Lu Zhenzu Chen Shuyun (Departement of Biology,Wuhan University)  
Fifteen strains producing high activity of alkaline pectinase were selected from the ninety-six alkalophilic bacteria isolated from alkaline soil.The range of the enzyme activity produced by above strains with shalking was 1467-2500 u·ml~(-1). Bacillus sp.LH 6 was determined as a stable obligate alkalophilic and alkline pectinase producing bacteria.This microorganism had good growth in pH 8-11.5 media,and no growth occurred below pH7.The culture conditions for the enzyme production was investigated.The organism was grown in the medium containing complex carbon source(2%(w/v)pectin and 2%(w/v) lactose)at an initial pH of 9.7 and 37℃,and the highest enzyme activity was obtained after 60 h cultivation with shalking.Glycine(0.2—1% w/v)was evidently effective to stimulate the production of the enzyme,and the average rate of increase in the enzyme activity produced by the organism was reached 34% at 0.5%(w/v).The investigation on some properties of the crude enzyme solution produced by Bacillus sp.LH 6 under above culure conditions indicated when the pectin was used as the substrate of the enzymes, the optimum pH and temperature for the enzyme activity was 11.5 and 60℃.
【Fund】: 国家自然科学基金资助课题
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