A Study of the Possible Anti-tumour Effect and Immunompetence of Pumpkin polysaccharide
Xu Guohua 1, Han Zhihong 1, Wu Yongfang 1, et al. (1 Wuhan Professional Medical College, Wuhan 430016, China)
Objective: To verify the anti tumour effects and immunompetence of polysaccharide isolated from pumpkin on mouse's transplantable tumour, and to develop a new anticancer drug from plant. Methods: Mice were transplanted with S 180 or Ehrlich's ascitic carcinoma. Drugs were given daily, by oral administration. Dosage of pumpkin polysaccharide was 0.4mL/day(10mg/mL) for seven days. On the eighth day animals were sacrificed and tumor nodules were removed from the transplant sites, and thymus were also removed. Parameters for this study were tumor and thymus weight, the former shown by inhibition rate and the latter by increase rate. Peripheral blood cell counts, as well as plasma total protein and albumin, SOD, MDA were determined. T test was used to analyse the results. Results: The inhibition rate of pumpkin polysaccharide for mice S 180 and EAC were 37.3% and 33.3% respectively and thymus increase rate were 8.3%. The effects of pumpkin polysccharide on peripheral blood counts were increasing numbers of peripheral leukocytes and lymphocytes and the pumpkin polysaccharide group was higher than Anliuru group (P0.01). There was no difference in erythrocytes and platelets counts and plasm total protein、 albumin between two groups. SOD activity increased and MDA content depressed, but there was no significant difference between two groups. Red blood cell immune function increased (P0.05). Conclusion: Pumpkin polysaccharide exhibits anti cancer activity and may increase peripheral leucocyte count and number of lymphocytes and enhance immunompetence. It is promising in adjuvant therapy. Further study is worth continuing.