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《Progress In Microbiology and Immunology》 1993-02
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Studies on the localization and serological response of the recombinant Vi 4072 in mice

Shen Yulin et al Lanzhou Institute of Biological Products, Lanzhou  
The Balb/c mice were fed with the recombinant strain Vi 4072 which was const ructed by genetic recombination using a aviru-tent strain of Sal.typhimurium as a receptor, and the geae coding for Vi was transformed to it. One dose was fed about 3 × 108CFU per m use. 4 days after the feeding at an interval 7, 14, 21, 28, 35, 42, 49, 56, 63 and 70days the mouse was sacrificed.The Peyers patch. liver and spleen were collected separately for identification whether the strain fed were present.The serum andemulsion of. the small intestine were examined if there wene Vi antibodies. The results showed that the isolates were positive last for 49 days in the spleen and Vi antibodies at least 70days in the serum.
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