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《Progress In Microbiology and Immunology》 1993-02
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Preparation of Anti-HBc Monoclonel Antibodies and Study on Clinical Application

Jiang Lin Xie Zhen Yang Zongmo Shen Xinliang Lanzhou Institute of Biological Prodcts Lanzhou 730046  
Five cell lines secreting anti-HBc monoclonal antibodies were established by using gene engineering expressed HBcAg as immunogen. The inhibition titers of the McAbs can be above ; :20000.Aatibodios remain the activity when the temperature up to 56℃for at least 8h and stable in pH 2.8-9.6.Using these antibodies, we made up the ELISA kit to detect anci-HBc in human plasma or serum, Tho reageats aro specific and sensitive and can pass the examination by standard of national institute for the control of pharmaceutical and biological products.
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