Study on Major Outer Membrane Proteins of Salmonella Typhimurium as a Protective Antigen
Zhao Yuewu et al(Department of Microbiology and Immunology, Henan Medical University, Zhengzuou 450052)
Major outer membrane proteins (MOMPs) from Salmonella typhimurium were extracted by using super sonication and Sephacral fine s-300, found to contain 0.2% lipopolyaccharide (LPS) approximately. MOMPs were shown 5 bands by SDS-PAGE. In MOMPs immunized BALB/c mice significant level of delayed-type hypersensitivity (DTH) response and interleukin-2(IL-2) production were detected. Their ablities of inducing protective immunity against challenge with the bacteria were studied in BALB/c mice. Active immunization with MOMPs induced 90% protection to a intraperitoneally (i, p) challenge with 500 LD50 of Salmonella typhimurium in BALB/c mice. In addition,33.3% cross-protection against challenge with 500 LD50 of Salmonella typhi was also achieved. Furthermore,We found that T cells from the MOMPs-immmunized mice could transfer the protection (71.4%) against Salmonella typhimurium to nonimmunized mice. These resultes suggest that STM-MOMPs can be a good protective antigen.
【CateGory Index】： R392-33