Proprety and Anti-Tumor Effect of Phenylalanine Ammonialyase from Sprouting Green Grams
Tang Xu; Zhou Guolin (Central Environmental Monitoring Station,Lanzhou Railway Bureau,Lanzhou 730000)
L-Phenylalanine ammonialyase was isolated and purified from sprouting green grams (Phaseolus radiatus L.).Both SDS-PAGE and IEF revealed a single stained protein band which corresponded to a subunit weight of 76 kD and to PI of 5. 45.Antitumor effect showed that the longer six PAI doses acted on murine leukemic lymphoblasts L1210 in vitro,the stronger the inhibitory effect was. The inhibitory rate of six PAL doses (0.2 U/ml, 1.0 U/ml ,2.0 U/ml,4.0 U/ml,6.0 U/ml,10.0 U/ml)at 72h was 25. 8% , 40.0%, 55.3 %, 72.6 %, 77.9%, 82.9% respectively.
【CateGory Index】： R341