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《Progress In Microbiology and Immunology》 2001-01
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Preparation,characterization and immunogenicity of conjugate vaccine composed of the O specific polysaccharide of S.flexneri 2a and tetanus toxoid

XIE Gui lin,FENG Jian jun,DU Lin,et al(Lanzhou Institute of Biological products,Lanzhou 730046)  
Lipolysaccharide (LPS) is both of the major toxic factor and the major protective antigen of Shigellae.It was considered that O specific polysaccharide (O SP) is the structure of protcetive antigenic.However,O SP alone can not elicit specific IgM and IgG both in human and animals because of its small molecular weight and simple structure which consisted of four repeat saccharide unit.The O SP of Shigella flexneri type 2a was purified and covalently bound to tetanus toxoid(TT) then formed a conjugate O SP TT.2.5μg of O SP or its conjugate was injected subcutaneously into NIH mouse,or 25μg iv injection a rabbit.Serum IgG and IgM antibodies against LPS of S.flexneri type 2a were measured by ELISA,and the bactericidal activity of anti. LPS antibody was measured by complement dependent bactericidal experiments.The results showed that the IgG and IgM antibodies are not detected in the serum of mice and rabbits serum immunized by O SP alone.A statistically significant rise in both of IgM and IgG could be elicited by O SP TT conjugate immunized mice and rabbits,the booster reactions occur after second and third injection as a result of the immunological memory response.Anti LPS antibodies in serum contain the bactericidal activity in confirmation with complement dependent bactericidal test in this study.
【CateGory Index】: R392-33
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHOU Lijuan,WANG Jiajun. Lanzhou Institute of Biological Products,Lanzhou 730046;Investgating biological activity of conjugate composed of the O-specific polysaccharide of Shigella dysenteriae type I bound to tetanus toxoid[J];Journal of Lanzhou Medical College;2004-04
2 WANG Yan,DU Lin,TIAN Yu zhen,et al(Lanzhou Institute of Biological Products,Lanzhou 730046);Preparation,characterization and immunogenicity of conjugate vaccine composed of the O-specific polysaccharide of S.sonnei and tetanus toxoid[J];Progress In Microbiology and Immunology;2002-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Jian-jie,WU Bin,CHENG Huan-chun (College of Animal science and vete rinar y Medicine Huazhong Agricultural Univers ity,Wuhan,430070,China);Progress of Research on the Toxigenic Pasteurella Multocida of Porcine Atrophic Rhinitis[J];Progress In Veterinary Medicine;2002-01
2 HUANG Shun-jun, WANG Xi-liang, LI Ming-yang, LUO De-yan, YANG You-rong, MA Li-juan ( Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences , Beijing 100071, China; Department of Horticulture and Landscape, Southwest Agricultural University, Chongqing 400716, China);High-level expression of Yersinia pestis F1-V fusion protein in Escherichia coli and its identification[J];Immunological Journal;2004-S1
3 NIU Ting-xian 1, LIU Ming-yuan 2, LU Qiang 2, FU Bao-quan 2, Pascal Boireau 3(1. Department of Laboratory Animal Science, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China; 2. Department of Military Veterinary, Quartermaster University of PLA, Changchun 130062, China; 3. UMR INRA-AFSSA-ENVA, Maison-Alfort, Paris 94703, France);Studies on protective immunity of Trichinella spiralis newborn larvae recombinant antigen in mice[J];Immunological Journal;2005-01
4 LIU Yuan-fa,WANG Xing-guo,JIN Qing-zhe(College of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China);Extraction of Flavone Analogues from Propolis Using Ultrasound[J];Food Science;2004-06
5 HUANG Jin-hai,WANG Ying-zhen,DING Bo-liang,ZHANG Jian,WANG Dong,YAN Ming-hua, LI Xiu-li(Tianjin Institute of Animal Husbandry and Veterinary Medicine,Tianjin 300112,China);Development of ELISA for Detecting Antibodies to Egg Drop Syndrome-76 Virus[J];Tianjin Agricultural Sciences;2004-02
6 WANG Yan,DU Lin,TIAN Yu zhen,et al(Lanzhou Institute of Biological Products,Lanzhou 730046);Preparation,characterization and immunogenicity of conjugate vaccine composed of the O-specific polysaccharide of S.sonnei and tetanus toxoid[J];Progress In Microbiology and Immunology;2002-02
7 CUI Bao an,YANG Ming fan,LI Rui fang,ZHANG Su mei,WANG Xue bin,WANG Li (College of Animal Husbandry and Veterinary Medicine,Henan Agric Univ,Zhengzhou 450002,China);Indirect ELISA for detection of antibody to PRV inactirated vaccine[J];Animal Husbandry & Veterinary Medicine;2003-01
8 Xu Chongbo,Zhu Ping,Yiao Xiangyan,Gan Yonghua, Feng Shuzhang,Ma Conglin and Meng Ruiqi (The Military Veterinary Institute,University of Agriculture and Animal Sciences of PLA,Changchun 130062);THE IMMUNOGENICITY OF THE EXPRESSED PRODUCT OF GENETIC ENGINEERING STRAIN EXPRESSING THE ALPHA TOXIN OF CLOSTRIDIUM PERFRINGENS[J];ANIMAL HUSBANDRY & VETERINARY MEDICINE;1998-06
9 XU Qian, GUO Hua, YE Huai_yi (College of Food and Medicine Engineering, Harbin Commercial University, Harbin 150076,China);Study of the Two-step-ethanol-leaching Method of Propolis[J];Applied Science and Technology;2002-07
10 GUAN Yu, SHEN Zhi-qiang, LIU Jin-shan, PANG Wan-yong,WANG Yan,MO Ling, (Shandong Binzhou Insititute of Animal Science and Veterinary Medicine,Shandong Research & Development Center of Veterinary Propolis Vaccine, Binzhou 256618,China);The application of 16S rRNA gene sequence analysis in the identification of avian pasteurella multocida[J];Chinese Journal of Preventive Veterinary Medicine;2003-05
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