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《Progress In Microbiology and Immunology》 2001-01
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Study on large scale production of mumps vaccine with 15 L rollig botlle

LIU Xiao min,ZHANG Jin feng,CHEN Jing cai,et al.(National Vaccine & Serum Institute,Beijing,100024)  
The optimal condition of preparation of mumps vaccine with 15 L rolling bottle is reported.The mean titers were 6.25 LogCCID 50 /ml when the cells and virus were simutaneously inoculated and incubated at 33℃ for five days.After the cell monolayer were formed,which were infected by the virus,the mean titer were 4.85 LogCCID 50 /ml.The chick embryo cells were infected with the virus at multiplicity of infection(MOI)of 2.3×10 -4 the virus titer may reach to 6.5 LogCCID 50 /ml.The virus suspension were harvested two times at 100 h and 120 h at the peak of virus titers,the titer of them were 6.00 and 6.50 LogCCID 50 /ml respectively.The yield of the vaccine were 4500 ml and 9000 ml per bottle without significant changes in their titers.The vaccine in liquid state were stored at 2 10℃ for 14 days and the titer had dropped from 5.75 to 4.75 LogCCID 50 /ml.While the vaccine was stored at -18℃ and the titer had slowly declined from 6.38 to 5.00 LogCCID 50 /ml.
【CateGory Index】: R392-33
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